miRBase entry: dme-mir-277

Stem-loop dme-mir-277

Drosophila melanogaster dme-mir-277 precursor miRNA
Gene family
MIPF0000156; mir-277

Literature search
24 open access papers mention dme-mir-277
(222 sentences)


3084746 reads, 22508 reads per million, 49 experiments

----   a g        c  -         -            c  aaa u 
    uug a guuuuggg ug CGUGUCAGG AGUGCAUUUGCA ug   c a
    ||| | |||||||| || ||||||||| |||||||||||| ||   | u
    aac u caagaccu AC GCAUGGUCU UCACGUAAAUgu ac   g c
uucu   a g        u  A         A            -  gaa u 

Annotation confidence High
Do you think this miRNA is real?
miR-277 was reported independently in references [1] and [2]. Computational prediction followed by northern blotting confirmed that the strand containing the predicted miR is predominantly expressed [1]. Reference [2] confirmed the 5' end of the excised miRNA by cloning and reported a length distribution of 21-23 nt with 23 nt the most commonly expressed. Stark et al. [3] have predicted that miR-277 controls the pathway for valine, leucine and isoleucine degradation by downregulating many of its enzymes.

Genome context
chr3R: 10100022-10100121 [+]
Clustered miRNAs
2 other miRNAs are < 10 kb from dme-mir-277
Name Accession Chromosome Start End Strand Confidence

Database links

Mature dme-miR-277-3p

Accession MIMAT0000338
Description Drosophila melanogaster dme-miR-277-3p mature miRNA
Evidence experimental
Northern [1], cloned [2], 454 [4-5], Illumina [5]
Database links
Predicted targets

Mature dme-miR-277-5p

Accession MIMAT0020804
Description Drosophila melanogaster dme-miR-277-5p mature miRNA
Evidence not_experimental
Database links


  1. PubMed ID: 12919683
    The small RNA profile during Drosophila melanogaster development
    "Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T"
    "Dev Cell (2003) 5:337-350

  2. PubMed ID: 17989254
    Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
    "Genome Res (2007) 17:1850-1864

  3. PubMed ID: 17989255
    Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
    "Genome Res (2007) 17:1865-1879

  4. PubMed ID: 14691535
    Identification of Drosophila MicroRNA targets
    "Stark A, Brennecke J, Russell RB, Cohen SM"
    "PLoS Biol (2003) 1:E60

  5. PubMed ID: 12844358
    Computational identification of Drosophila microRNA genes
    "Lai EC, Tomancak P, Williams RW, Rubin GM"
    "Genome Biol (2003) 4:R42