Accession
MI0000373
Description
Drosophila melanogaster
dme-mir-124 precursor miRNA
Summary
Caution, this is an AI generated summary based on literature. This may have errors. ?
Dme-mir-124 is a microRNA that has been selected based on data generated in the laboratory and its orthology with human miRNAs [PMC5090246]. It has been found to play a role in male sexual differentiation in Drosophila melanogaster by targeting a sex-specific splicing factor in the sex determination pathway [PMC8966896]. Mutant male flies with dme-mir-124 exhibit aberrant pheromone levels, leading to reduced mating success and receptivity by females, as well as increased male-male courtship [PMC8966896]. Dme-mir-124 is similar to vertebrate miR-124a and shows a similar expression pattern to dme-miR-263a and -263b, with trough levels during mid-day followed by increases during the early to late-night in wildtype flies and constantly elevated levels in the cyc0 mutant [PMC2263044]. Among the 78 miRNAs probed for expression profiling, dme-miR263a and -263b displayed strong evidence of circadian regulation, while dme-mir-124 showed possible weak cycling [PMC2263044]. The low apparent sensitivity of sensors for dme-miR-1000, hsa-miR-223–3p, dme-miR-14, and dme-mir-124 was attributed to high background fluorescence in the absence of these miRNAs [PMC4889923].
Literature search
19 open access papers mention dme-mir-124
(339 sentences)
(339 sentences)
Sequence
295935
reads,
23619
reads per million, 48 experiments
ucauuugguacguuuuucuccuGGUAUCCACUGUAGGCCUAUAUGuauuuccaccaUAAGGCACGCGGUGAAUGCCAAGagcgaacgcaguucuacaaau
..........(((((..(((.((((((.((((((..((((.((((.........))))))))..)))))).)))))).))).))))).............
..........(((((..(((.((((((.((((((..((((.((((.........))))))))..)))))).)))))).))).))))).............
Structure
---ucauuuggua uu c C AG A uau
cguuu cuc uGGUAU CACUGU GCCU UAUG u
||||| ||| |||||| |||||| |||| |||| u
gcaag gaG ACCGUA GUGGCG CGGA AUac c
uaaacaucuugac -c A A CA - cac
Annotation confidence
High
Do you think this miRNA is real?
Comments
miR-124 was reported independently in references [1] and [2]. Computational prediction followed by northern blotting confirmed that the strand containing the predicted miR is predominantly expressed [1]. Reference [2] confirmed the 5' end of the excised miRNA by cloning and reported a length distribution of 19-23 nt with 23 nt the most commonly expressed.
Genome context
chr2L: 17566370-17566469 [+]
Clustered miRNAs
1 other miRNA is < 10 kb from dme-mir-124
| Name | Accession | Chromosome | Start | End | Strand | Confidence |
|---|
Database links
Mature dme-miR-124-3p
| Accession | MIMAT0000351 |
| Description | Drosophila melanogaster dme-miR-124-3p mature miRNA |
| Sequence | 57 - UAAGGCACGCGGUGAAUGCCAAG - 79 |
| Evidence |
experimental
cloned [2], Northern [2-3], 454 [4-5], Illumina [5] |
| Database links |
|
| Predicted targets |
|
Mature dme-miR-124-5p
| Accession | MIMAT0020813 |
| Description | Drosophila melanogaster dme-miR-124-5p mature miRNA |
| Sequence | 23 - GGUAUCCACUGUAGGCCUAUAUG - 45 |
| Evidence | not_experimental |
| Database links |
|
References
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