miRBase entry: dme-mir-124

Stem-loop dme-mir-124

Drosophila melanogaster dme-mir-124 precursor miRNA
Gene family
MIPF0000021; mir-124

Caution, this is an AI generated summary based on literature. This may have errors. ?

Dme-mir-124 is a microRNA that has been selected based on data generated in the laboratory and its orthology with human miRNAs [PMC5090246]. It has been found to play a role in male sexual differentiation in Drosophila melanogaster by targeting a sex-specific splicing factor in the sex determination pathway [PMC8966896]. Mutant male flies with dme-mir-124 exhibit aberrant pheromone levels, leading to reduced mating success and receptivity by females, as well as increased male-male courtship [PMC8966896]. Dme-mir-124 is similar to vertebrate miR-124a and shows a similar expression pattern to dme-miR-263a and -263b, with trough levels during mid-day followed by increases during the early to late-night in wildtype flies and constantly elevated levels in the cyc0 mutant [PMC2263044]. Among the 78 miRNAs probed for expression profiling, dme-miR263a and -263b displayed strong evidence of circadian regulation, while dme-mir-124 showed possible weak cycling [PMC2263044]. The low apparent sensitivity of sensors for dme-miR-1000, hsa-miR-223–3p, dme-miR-14, and dme-mir-124 was attributed to high background fluorescence in the absence of these miRNAs [PMC4889923].

Literature search
19 open access papers mention dme-mir-124
(339 sentences)


295935 reads, 23619 reads per million, 48 experiments

---ucauuuggua     uu   c      C      AG    A    uau 
             cguuu  cuc uGGUAU CACUGU  GCCU UAUG   u
             |||||  ||| |||||| ||||||  |||| ||||   u
             gcaag  gaG ACCGUA GUGGCG  CGGA AUac   c
uaaacaucuugac     -c   A      A      CA    -    cac 

Annotation confidence High
Do you think this miRNA is real?
miR-124 was reported independently in references [1] and [2]. Computational prediction followed by northern blotting confirmed that the strand containing the predicted miR is predominantly expressed [1]. Reference [2] confirmed the 5' end of the excised miRNA by cloning and reported a length distribution of 19-23 nt with 23 nt the most commonly expressed.

Genome context
chr2L: 17566370-17566469 [+]
Clustered miRNAs
1 other miRNA is < 10 kb from dme-mir-124
Name Accession Chromosome Start End Strand Confidence

Database links

Mature dme-miR-124-3p

Accession MIMAT0000351
Description Drosophila melanogaster dme-miR-124-3p mature miRNA
Evidence experimental
cloned [2], Northern [2-3], 454 [4-5], Illumina [5]
Database links
Predicted targets

Mature dme-miR-124-5p

Accession MIMAT0020813
Description Drosophila melanogaster dme-miR-124-5p mature miRNA
Evidence not_experimental
Database links


  1. PubMed ID: 12812784
    Temporal regulation of microRNA expression in Drosophila melanogaster mediated by hormonal signals and broad-Complex gene activity
    "Sempere LF, Sokol NS, Dubrovsky EB, Berger EM, Ambros V"
    "Dev Biol (2003) 259:9-18

  2. PubMed ID: 12919683
    The small RNA profile during Drosophila melanogaster development
    "Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T"
    "Dev Cell (2003) 5:337-350

  3. PubMed ID: 17989254
    Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
    "Genome Res (2007) 17:1850-1864

  4. PubMed ID: 17989255
    Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
    "Genome Res (2007) 17:1865-1879

  5. PubMed ID: 12844358
    Computational identification of Drosophila microRNA genes
    "Lai EC, Tomancak P, Williams RW, Rubin GM"
    "Genome Biol (2003) 4:R42