miRBase entry: dme-mir-iab-4

Stem-loop dme-mir-iab-4


Accession
MI0000432
Description
Drosophila melanogaster dme-mir-iab-4 precursor miRNA


Sequence

8957 reads, 799 reads per million, 45 experiments
ucguaaACGUAUACUGAAUGUAUCCUGAguguauccuaucCGGUAUACCUUCAGUAUACGUAACacga
((((..((((((((((((.((((((.((..(....)..)).)).)))).))))))))))))...))))

Structure
    -aa            U    -  U  gu u 
ucgu   ACGUAUACUGAA GUAU CC GA  g a
||||   |||||||||||| |||| || ||  |  
agca   UGCAUAUGACUU CAUA GG cu  c u
    CAA            C    U  C  au c 


Annotation confidence High
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Comments
iab-4 is a transcript of the Bithorax complex which has been previously shown to contribute to proper formation of abdominal segments [2]. Reference [1] identified a miRNA gene within the transcript and mapped the ends of the mature miR sequence by cloning. Excised miR sequences were identified from both arms of the precursor and are named miR-iab-4-5p (5') and miR-iab-4-3p (3') as too few sequences were cloned to decide on which was predominantly expressed.

Genome context
chr3R: 16856275-16856342 [+]
Clustered miRNAs
1 other miRNA is < 10 kb from dme-mir-iab-4
Name Accession Chromosome Start End Strand Confidence




Database links

Mature dme-miR-iab-4-5p

Accession MIMAT0000412
Description Drosophila melanogaster dme-miR-iab-4-5p mature miRNA
Sequence 7 - ACGUAUACUGAAUGUAUCCUGA - 28
Evidence experimental
cloned [1], 454 [3-4], Illumina [4]
Database links
Predicted targets

Mature dme-miR-iab-4-3p

Accession MIMAT0000413
Description Drosophila melanogaster dme-miR-iab-4-3p mature miRNA
Sequence 41 - CGGUAUACCUUCAGUAUACGUAAC - 64
Evidence experimental
cloned [1], 454 [3-4], Illumina [4]
Database links
Predicted targets

References

  1. PubMed ID: 12919683
    The small RNA profile during Drosophila melanogaster development
    "Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T"
    "Dev Cell (2003) 5:337-350

  2. PubMed ID: 17989254
    Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
    "Genome Res (2007) 17:1850-1864

  3. PubMed ID: 17989255
    Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
    "Genome Res (2007) 17:1865-1879

  4. PubMed ID: 11504843
    The evolution of controlled multitasked gene networks: the role of introns and other noncoding RNAs in the development of complex organisms
    "Mattick JS, Gagen MJ"
    "Mol Biol Evol (2001) 18:1611-1630