Stem-loop mmu-mir-328

Accession

MI0000603

Symbol

MGI: Mir328

Description

Mus musculus mmu-mir-328 precursor miRNA mir-328

Gene
family

RF00772; mir-328

Literature search

34 open access papers mention mmu-mir-328
(179 sentences)

Sequence

31960 reads, 125 reads per million, 106 experiments

cugucucggagccuggggcaGGGGGGCAGGAGGGGCUCAGGGagaaaguaucuacagcccCUGGCCCUCUCUGCCCUUCCGUccccuguccccaagu
..........((.(((((((((((((((((.(((((.((((((((.....))).....)))))))))).)))))).......)))))).))))).))

Structure

cugucucgga  c     -      -------      A     U     -----   a 
          gc ugggg caGGGG       GGCAGG GGGGC CAGGG     aga a
          || ||||| ||||||       |||||| ||||| |||||     ||| g
          ug acccc gucccc       CCGUCU UCCCG GUCcc     ucu u
----------  a     u      UGCCUUC      C     -     cgaca   a 

Annotation confidence Medium

Comments

Kim et al. cloned 40 new miRNAs from rat E18 primary cortical neurons [1]. This mouse miRNA is predicted based on homology to the verified rat sequence (MIR:MI0000602) - its expression was later independently verified in mouse [2].

Genome context

chr8: 105308364-105308460 [-]

Mature mmu-miR-328-5p

• Accession: MIMAT0017030
• Description: Mus musculus mmu-miR-328-5p mature miRNA
• Sequence: 21 - GGGGGGCAGGAGGGGCUCAGGG - 42
• Evidence: experimental ; Illumina [5]

Mature mmu-miR-328-3p

• Accession: MIMAT0000565
• Description: Mus musculus mmu-miR-328-3p mature miRNA
• Sequence: 61 - CUGGCCCUCUCUGCCCUUCCGU - 82
• Evidence: experimental ; cloned [2-3], Illumina [4-5]

References

1. PubMed ID: 17604727
   A mammalian microRNA expression atlas based on small RNA library sequencing
   "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
   "Cell (2007) 129:1401-1414


2. PubMed ID: 15538371
   A pancreatic islet-specific microRNA regulates insulin secretion
   "Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE, Pfeffer S, Tuschl T, Rajewsky N, Rorsman P, Stoffel M"
   "Nature (2004) 432:226-230


3. PubMed ID: 20215419
   MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing
   "Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A"
   "Mol Hum Reprod (2010) 16:463-471


4. PubMed ID: 20413612
   Mammalian microRNAs: experimental evaluation of novel and previously annotated genes
   "Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP"
   "Genes Dev (2010) 24:992-1009


5. PubMed ID: 14691248
   Identification of many microRNAs that copurify with polyribosomes in mammalian neurons
   "Kim J, Krichevsky A, Grad Y, Hayes GD, Kosik KS, Church GM, Ruvkun G"
   "Proc Natl Acad Sci U S A (2004) 101:360-365