miRBase entry: mmu-mir-25

Stem-loop mmu-mir-25

MGI: Mir25
Mus musculus mmu-mir-25 precursor miRNA
Gene family
MIPF0000013; mir-25

Caution, this is an AI generated summary based on literature. This may have errors. ?

Mmu-mir-25 is a microRNA that is downregulated in DIO mice [PMC4571067]. It is also found to be more prominent and may play crucial roles in the regulatory network [PMC3692539]. Mmu-mir-25 has been extensively expressed during superovulation [PMC4499447]. It is a member of the miR-106b~25 cluster and is located in the 13th intron of the MCM7 gene [PMC7803573]. Overexpression of mmu-mir-25 has been shown to enhance iPSC generation, while knockdown of mmu-mir-25 decreases reprogramming efficiency [PMC8946776]. The precursor sequence of mmu-mir-25, along with upstream and downstream flanking regions, has been amplified from mouse genomic DNA [PMC8355162]. Inhibition of mmu-mir-25 increases reactive oxygen species levels and overexpression of Nox4 [PMC8914318]. Mmu-mir-25 has also been found to be decreased in mice with diabetic peripheral neuropathy [PMC8914318]. Trizol purified RNA was used to clean up miRNA samples, including mmu-mir-25, for expression profiling experiments using Illumina BeadChips [PMC3422281].

In summary, mmu-mir-25 is a downregulated microRNA in DIO mice that may play crucial roles in regulatory networks. It is expressed during superovulation and its overexpression enhances iPSC generation. Inhibition of mmu-mir-25 increases reactive oxygen species levels. Additionally, it has been found to be decreased in mice with diabetic peripheral neuropathy. The precursor sequence of mmu-miR-106b~25 cluster member mmu-miR-25 has been amplified from mouse genomic DNA for further analysis. Trizol purified RNA was used for expression profiling experiments involving mmu-mir-25. [PMC4571067] [PMC3692539] [PMC4499447] [PMC7803573] [PMC8946776] [PMC8355162] [PMC8914318] [PMC3422281].

Literature search
91 open access papers mention mmu-mir-25
(506 sentences)


3342880 reads, 8777 reads per million, 107 experiments

    a      ag    G     UU G     UG    acg 
ggcc guguug  AGGC GAGAC  G GCAAU  Cugg   c
|||| ||||||  |||| |||||  | |||||  ||||    
ccgg cgugac  UCUG CUCUG  C CGUUA  gguc   u
    c      AG    G     UU A     Cg    ccg 

Annotation confidence High
Do you think this miRNA is real?
Mouse mir-25 is predicted [3] based on homology to a cloned miR from human (MIR:MI0000082) [1]. Its expression has been later independently verified in mouse [2,4].

Genome context
chr5: 138165321-138165404 [-]
Clustered miRNAs
2 other miRNAs are < 10 kb from mmu-mir-25
Name Accession Chromosome Start End Strand Confidence

Database links

Mature mmu-miR-25-5p

Accession MIMAT0017049
Description Mus musculus mmu-miR-25-5p mature miRNA
Evidence experimental
454 [6], Illumina [7]
Database links
Predicted targets

Mature mmu-miR-25-3p

Accession MIMAT0000652
Description Mus musculus mmu-miR-25-3p mature miRNA
Evidence experimental
cloned [2,4], Illumina [5,7]
Database links
Predicted targets


  1. PubMed ID: 11679670
    Identification of novel genes coding for small expressed RNAs
    "Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T"
    "Science (2001) 294:853-858

  2. PubMed ID: 17604727
    A mammalian microRNA expression atlas based on small RNA library sequencing
    "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
    "Cell (2007) 129:1401-1414

  3. PubMed ID: 15538371
    A pancreatic islet-specific microRNA regulates insulin secretion
    "Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE, Pfeffer S, Tuschl T, Rajewsky N, Rorsman P, Stoffel M"
    "Nature (2004) 432:226-230

  4. PubMed ID: 20215419
    MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing
    "Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A"
    "Mol Hum Reprod (2010) 16:463-471

  5. PubMed ID: 20413612
    Mammalian microRNAs: experimental evaluation of novel and previously annotated genes
    "Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP"
    "Genes Dev (2010) 24:992-1009

  6. PubMed ID: 15634332
    New human and mouse microRNA genes found by homology search
    "Weber MJ"
    "FEBS J (2005) 272:59-73

  7. PubMed ID: 20668074
    Identification and analysis of expression of novel microRNAs of murine gammaherpesvirus 68
    "Zhu JY, Strehle M, Frohn A, Kremmer E, Hofig KP, Meister G, Adler H"
    "J Virol (2010) 84:10266-10275