Stem-loop mmu-mir-412

Accession

MI0001164

Symbol

MGI: Mir412

Description

Mus musculus mmu-mir-412 precursor miRNA mir-412

Gene
family

RF00762; mir-412

Literature search

7 open access papers mention mmu-mir-412
(36 sentences)

Sequence

3167 reads, 35 reads per million, 48 experiments

ggguaugggacggaUGGUCGACCAGCUGGAAAGUAAUuguuucuaauguacUUCACCUGGUCCACUAGCCGucggugccc
((((((..(((((.((((.((((((.(((..((((((((....)))).))))))).)))))).)))).))))).))))))

Structure

      gg     a    C      C   AA    -    u 
ggguau  gacgg UGGU GACCAG UGG  AGUA AUug u
||||||  ||||| |||| |||||| |||  |||| ||||  
cccgug  cuGCC AUCA CUGGUC ACU  Ucau uaau u
      -g     G    C      C   --    g    c 

Annotation confidence High

Comments

Seitz et al. predicted a cluster of 40 miRNAs in the imprinted human 14q32 domain, and confirmed the expression of a subset by Northern blot or primer extension in mouse [1]. The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [3]. The 5' end of the miRNA may be offset with respect to previous annotations.

Genome context

chr12: 109743289-109743368 [+]

Clustered miRNAs

13 other miRNAs are < 10 kb from mmu-mir-412

Mature mmu-miR-412-5p

• Accession: MIMAT0017173
• Description: Mus musculus mmu-miR-412-5p mature miRNA
• Sequence: 15 - UGGUCGACCAGCUGGAAAGUAAU - 37
• Evidence: experimental ; Illumina [5]

Mature mmu-miR-412-3p

• Accession: MIMAT0001094
• Description: Mus musculus mmu-miR-412-3p mature miRNA
• Sequence: 52 - UUCACCUGGUCCACUAGCCG - 71
• Evidence: experimental ; PCR [1], cloned [2-3], Illumina [4-5]

References

1. PubMed ID: 17604727
   A mammalian microRNA expression atlas based on small RNA library sequencing
   "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
   "Cell (2007) 129:1401-1414


2. PubMed ID: 20215419
   MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing
   "Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A"
   "Mol Hum Reprod (2010) 16:463-471


3. PubMed ID: 20413612
   Mammalian microRNAs: experimental evaluation of novel and previously annotated genes
   "Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP"
   "Genes Dev (2010) 24:992-1009


4. PubMed ID: 15310658
   A large imprinted microRNA gene cluster at the mouse Dlk1-Gtl2 domain
   "Seitz H, Royo H, Bortolin ML, Lin SP, Ferguson-Smith AC, Cavaille J"
   "Genome Res (2004) 14:1741-1748


5. PubMed ID: 16274478
   Identification of clustered microRNAs using an ab initio prediction method
   Sewer A, Paul N, Landgraf P, Aravin A, Pfeffer S, Brownstein MJ, Tuschl T, van Nimwegen E, Zavolan M
   BMC Bioinformatics (2005) 6:267