Stem-loop ath-MIR414

Accession

MI0001425

Description

Arabidopsis thaliana ath-MIR414 precursor miRNA

Literature search

10 open access papers mention ath-MIR414
(41 sentences)

Sequence

1664 reads, 14.0 reads per million, 166 experiments

ucaucauuaucaucaucaucauauucaucuucaucaucaucguCAUCAUCAUCAUCAUCAUCguaugagaagauagagaagagugAGAGUAUGAGAUUUGAGUUGAGA
...(((...(((..(((.((((((((.(((((.((.(((((.((.(((((............).)))))).))).)))).))).))))))))))))).)))..)))..

Structure

uca   uua   uc   a        a  -   a  a  -   g  A    - AUCAU 
   uca   uca  auc ucauauuc uc uuc uc uc auc uC UCAU C     C
   |||   |||  ||| |||||||| || ||| || || ||| || |||| |      
   AGU   AGU  UAG AGUAUGAG Ag gag ag ag uag ag agua g     A
-AG   -UG   -U   -        -  u   a  -  a   a  -    u CUACU 

Annotation confidence Low

Comments

The status of this sequence as a miRNA has been questioned on the basis of lack of conservation in genomes other than Arabidopsis and rice, moderately poor precursor hairpin structure, lack of identified targets, and low Northern blot signal [2]. This sequence may therefore be removed in subsequent data releases.

Genome context

chr1: 25137456-25137563 [-]

Mature ath-miR414-5p

• Accession: MIMAT0001322
• Description: Arabidopsis thaliana ath-miR414-5p mature miRNA
• Sequence: 44 - CAUCAUCAUCAUCAUCAUC - 62
• Evidence: experimental ; Northern [1]

Mature ath-miR414-3p

• Accession: MIMAT0053847
• Description: Arabidopsis thaliana ath-miR414-3p mature miRNA
• Sequence: 86 - AGAGUAUGAGAUUUGAGUUGAGA - 108
• Evidence: experimental ; unknown

References

1. PubMed ID: 15345049
   Prediction and identification of Arabidopsis thaliana microRNAs and their mRNA targets
   Wang XJ, Reyes JL, Chua NH, Gaasterland T
   Genome Biol (2004) 5:R65


2. PubMed ID: 16669754
   MicroRNAS and their regulatory roles in plants
   "Jones-Rhoades MW, Bartel DP, Bartel B"
   "Annu Rev Plant Biol (2006) 57:19-53