Stem-loop ppy-mir-10a

Accession

MI0002789

Description

Pongo pygmaeus ppy-mir-10a precursor miRNA

Sequence

gaucugucugucuucuguauaUACCCUGUAGAUCCGAAUUUGUGuaaggaauuuuguggucacaaauucguaucuaggggaauauguaguugacauaaacacuccgcucg
....(((.((((..((((((((.((((.(((((.((((((((((................)))))))))).))))))))).))))))))..))))...))).........

Structure

-----gauc   --c    uu        A    G     C          uaaggaa 
         ugu   uguc  cuguauaU CCCU UAGAU CGAAUUUGUG       u
         |||   ||||  |||||||| |||| ||||| ||||||||||        
         aca   acag  gauguaua gggg aucua gcuuaaacac       u
gcucgccuc   aau    uu        a    -     u          ugguguu 

Annotation confidence Not enough data

Comments

Berezikov et al. used primers designed from human miRNA gene flanking sequence to amplify miRNA precursor regions in primates [1]. The expression of the mature miRNA was not validated.

Genome context

NW_002963091.1: 1547447-1547556 [-]

Mature ppy-miR-10a

• Accession: MIMAT0002487
• Description: Pongo pygmaeus ppy-miR-10a mature miRNA
• Sequence: 22 - UACCCUGUAGAUCCGAAUUUGUG - 44
• Evidence: experimental ; Illumina [2]

References

1. PubMed ID: 15652478
   Phylogenetic shadowing and computational identification of human microRNA genes
   "Berezikov E, Guryev V, van de Belt J, Wienholds E, Plasterk RH, Cuppen E"
   "Cell (2005) 120:21-24


2. PubMed ID: 22453055
   Annotation of primate miRNAs by high throughput sequencing of small RNA libraries
   "Dannemann M, Nickel B, Lizano E, Burbano HA, Kelso J"
   "BMC Genomics (2012) 13:116