Stem-loop dre-mir-212

Accession

MI0003385

Description

Danio rerio dre-mir-212 precursor miRNA

Literature search

5 open access papers mention dre-mir-212
(57 sentences)

Sequence

250 reads, 30 reads per million, 10 experiments

gucagugcaucaauaccuuggcucuagacugcuuacugcuaaaugugucugaaaguacagUAACAGUCUACAGUCAUGGCUacugacgucuggc
(((((.((.(((.((((.(((((.(((((((.((((((...................))))))))))))).))))).)).)).))).)))))))

Structure

     u  a   a  -  u     c       c      cuaaaugu 
gucag gc uca ua cc uggcu uagacug uuacug        g
||||| || ||| || || ||||| ||||||| ||||||        u
cgguc ug agu aU GG ACUGA AUCUGAC AAUgac        c
     -  c   c  C  U     C       -      augaaagu 

Annotation confidence High

Comments

This miRNA was predicted by computational methods using conservation between human, mouse and Fugu rubripes sequences [1]. Expression of the excised miR was validated in zebrafish, and the 5' end mapped by PCR. The 3' end was not experimentally determined.

Genome context

chr15: 25897446-25897539 [+]

Clustered miRNAs

1 other miRNA is < 10 kb from dre-mir-212

Mature dre-miR-212

• Accession: MIMAT0003049
• Description: Danio rerio dre-miR-212 mature miRNA
• Sequence: 61 - UAACAGUCUACAGUCAUGGCU - 81
• Evidence: experimental ; PCR [1]

References

1. PubMed ID: 12624257
   Vertebrate microRNA genes
   "Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP"
   "Science (2003) 299:1540