Stem-loop mmu-mir-302d

Accession

MI0003718

Symbol

MGI: Mir302d

Description

Mus musculus mmu-mir-302d precursor miRNA mir-302

Gene
family

RF00668; mir-302

Literature search

76 open access papers mention mmu-mir-302d
(535 sentences)

Sequence

1214 reads, 48 reads per million, 19 experiments

ccuuuACUUUAACAUGGAGGCACUUGCUgugcauuuaaaaaUAAGUGCUUCCAUGUUUGAGUGUgg
((..(((((.((((((((((((((((...............)))))))))))))))).))))).))

Structure

  uu     U                CUgugc 
cc  uACUU AACAUGGAGGCACUUG      a
||  ||||| ||||||||||||||||      u
gg  GUGAG UUGUACCUUCGUGAAU      u
  -U     U                aaaaau 

Annotation confidence Medium

Comments

Mouse miR-302d was verified experimentally by Mineno et al using MPSS technology [1]. The MPSS protocol used provides 22nt sequences, but the true extents of the mature miRNA are not reliably obtained. The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [2]. The 5' end of the miRNA may be offset with respect to previous annotations.

Genome context

chr3: 127545624-127545689 [+]

Clustered miRNAs

4 other miRNAs are < 10 kb from mmu-mir-302d

Mature mmu-miR-302d-5p

• Accession: MIMAT0017225
• Description: Mus musculus mmu-miR-302d-5p mature miRNA
• Sequence: 6 - ACUUUAACAUGGAGGCACUUGCU - 28
• Evidence: experimental ; Illumina [3]

Mature mmu-miR-302d-3p

• Accession: MIMAT0003377
• Description: Mus musculus mmu-miR-302d-3p mature miRNA
• Sequence: 42 - UAAGUGCUUCCAUGUUUGAGUGU - 64
• Evidence: experimental ; MPSS [1], cloned [2], Illumina [3]

References

1. PubMed ID: 17604727
   A mammalian microRNA expression atlas based on small RNA library sequencing
   "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
   "Cell (2007) 129:1401-1414


2. PubMed ID: 20413612
   Mammalian microRNAs: experimental evaluation of novel and previously annotated genes
   "Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP"
   "Genes Dev (2010) 24:992-1009


3. PubMed ID: 16582102
   The expression profile of microRNAs in mouse embryos
   "Mineno J, Okamoto S, Ando T, Sato M, Chono H, Izu H, Takayama M, Asada K, Mirochnitchenko O, Inouye M, Kato I"
   "Nucleic Acids Res (2006) 34:1765-1771