miRBase entry: mmu-mir-143

Stem-loop mmu-mir-143


Accession
MI0000257
Symbol
MGI: Mir143
Description
Mus musculus mmu-mir-143 precursor miRNA
Gene family
MIPF0000094; mir-143

Literature search
177 open access papers mention mmu-mir-143
(1111 sentences)

Sequence

7547207 reads, 19717 reads per million, 99 experiments
ccugaGGUGCAGUGCUGCAUCUCUGGucaguugggagucUGAGAUGAAGCACUGUAGCUCagg
((((((.(((((((((.((((((.((((......)).)).)))))).))))))))).))))))

Structure
      G         G      U  -  ag 
ccugaG UGCAGUGCU CAUCUC GG uc  u
|||||| ||||||||| |||||| || ||   
ggaCUC AUGUCACGA GUAGAG cu ag  u
      G         A      U  g  gg 


Annotation confidence High
Do you think this miRNA is real?
Comments
Expression of this miRNA in mouse was independently verified in heart and brain [1], embryonic stem cells [2] and testes [3]. The predominant miRNA cloned by Langraf et al. has a 3' terminal U residue, which is incompatible with the genome sequence [4].

Genome context
chr18: 61649196-61649258 [-]
Clustered miRNAs
1 other miRNA is < 10 kb from mmu-mir-143
Name Accession Chromosome Start End Strand Confidence




Database links

Mature mmu-miR-143-5p

Accession MIMAT0017006
Description Mus musculus mmu-miR-143-5p mature miRNA
Sequence 6 - GGUGCAGUGCUGCAUCUCUGG - 26
Evidence experimental
454 [6], Illumina [7]
Database links
Predicted targets

Mature mmu-miR-143-3p

Accession MIMAT0000247
Description Mus musculus mmu-miR-143-3p mature miRNA
Sequence 40 - UGAGAUGAAGCACUGUAGCUC - 60
Evidence experimental
cloned [1-4], Illumina [5,7]
Database links
Predicted targets

References

  1. PubMed ID: 17604727
    A mammalian microRNA expression atlas based on small RNA library sequencing
    "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
    "Cell (2007) 129:1401-1414

  2. PubMed ID: 12919684
    Embryonic stem cell-specific MicroRNAs
    "Houbaviy HB, Murray MF, Sharp PA"
    "Dev Cell (2003) 5:351-358

  3. PubMed ID: 12007417
    Identification of tissue-specific microRNAs from mouse
    "Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T"
    "Curr Biol (2002) 12:735-739

  4. PubMed ID: 16766679
    Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes
    "Watanabe T, Takeda A, Tsukiyama T, Mise K, Okuno T, Sasaki H, Minami N, Imai H"
    "Genes Dev (2006) 20:1732-1743

  5. PubMed ID: 20215419
    MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing
    "Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A"
    "Mol Hum Reprod (2010) 16:463-471

  6. PubMed ID: 20413612
    Mammalian microRNAs: experimental evaluation of novel and previously annotated genes
    "Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP"
    "Genes Dev (2010) 24:992-1009

  7. PubMed ID: 20668074
    Identification and analysis of expression of novel microRNAs of murine gammaherpesvirus 68
    "Zhu JY, Strehle M, Frohn A, Kremmer E, Hofig KP, Meister G, Adler H"
    "J Virol (2010) 84:10266-10275