Stem-loop aae-mir-305

Accession

MI0013459

Description

Aedes aegypti aae-mir-305 precursor miRNA

Literature search

2 open access papers mention aae-mir-305
(8 sentences)

Sequence

35307 reads, 1830 reads per million, 2 experiments

aaugucacaugucuAUUGUACUUCAUCAGGUGCUCUGGuuguuuucgauaccCGGCACAUGUUGGAGUACACUUAaugugcugacagu
..((((((((((..(.(((((((((.((.(((((..(((..........))).))))).)).))))))))).)..))))).)))))..

Structure

aa     -     cu U         U  G     CU   uguu 
  uguca caugu  A UGUACUUCA CA GUGCU  GGu    u
  ||||| |||||  | ||||||||| || |||||  |||     
  acagu gugua  U ACAUGAGGU GU CACGG  cca    u
ug     c     AU C         U  A     -C   uagc 

Annotation confidence High

Comments

This miRNA was identified independently in Aedes aegypti [1] and in the closely related Aedes albopictus, for which there was no genome sequence [2]. The latter reads were therefore also mapped to the Aedes aegypti genome [2].

Genome context

supercont1.24: 495587-495674 [+]

Clustered miRNAs

1 other miRNA is < 10 kb from aae-mir-305

Mature aae-miR-305-5p

• Accession: MIMAT0014249
• Description: Aedes aegypti aae-miR-305-5p mature miRNA
• Sequence: 15 - AUUGUACUUCAUCAGGUGCUCUGG - 38
• Evidence: experimental ; 454 [1], Illumina [2]

Mature aae-miR-305-3p

• Accession: MIMAT0014250
• Description: Aedes aegypti aae-miR-305-3p mature miRNA
• Sequence: 53 - CGGCACAUGUUGGAGUACACUUA - 75
• Evidence: experimental ; 454 [1], Illumina [2]

References

1. PubMed ID: 19961592
   Direct sequencing and expression analysis of a large number of miRNAs in Aedes aegypti and a multi-species survey of novel mosquito miRNAs
   Li S, Mead EA, Liang S, Tu Z
   BMC Genomics (2009) 10:581


2. PubMed ID: 20167119
   Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus
   Skalsky RL, Vanlandingham DL, Scholle F, Higgs S, Cullen BR
   BMC Genomics (2010) 11:119