miRBase entry: aae-mir-309a-2

Stem-loop aae-mir-309a-2


Accession
MI0013478
Description
Aedes aegypti aae-mir-309a-2 precursor miRNA
Gene family
MIPF0000140; mir-3

Literature search
2 open access papers mention aae-mir-309a-2
(3 sentences)

Sequence

2 reads, 0 reads per million, 2 experiments
uccaccgaauguuaugcgacuaacuucguucaguuuggugucauacgaagguuuccaguuucaUCACUGGGCAAAGUUUGUCGCauaaaug
...........(((((((((.(((((.(((((((..((((..((..(((...)))..))..))))))))))).))))).)))))))))...

Structure
uccaccgaaug         u     c       uu    uc  ac   g 
           uuaugcgac aacuu guucagu  ggug  au  gaa  
           ||||||||| ||||| |||||||  ||||  ||  ||| g
           aauaCGCUG UUGAA CGGGUCA  CUac  ug  cuu  
--------gua         U     A       --    uu  ac   u 


Annotation confidence Not enough data
Do you think this miRNA is real?
Comments
This miRNA was experimentally identified in the closely related Aedes albopictus in [2], for which there was no genome sequence, and therefore mapped to the Aedes aegypti genome. Li et al. also validate the expression in A. aegypti [1].

Genome context
supercont1.602: 94025-94115 [+]
Clustered miRNAs
1 other miRNA is < 10 kb from aae-mir-309a-2
Name Accession Chromosome Start End Strand Confidence




Database links

Mature aae-miR-309a

Accession MIMAT0014272
Description Aedes aegypti aae-miR-309a mature miRNA
Sequence 64 - UCACUGGGCAAAGUUUGUCGC - 84
Evidence experimental
Illumina [1], 454 [2]

References

  1. PubMed ID: 19961592
    Direct sequencing and expression analysis of a large number of miRNAs in Aedes aegypti and a multi-species survey of novel mosquito miRNAs
    Li S, Mead EA, Liang S, Tu Z
    BMC Genomics (2009) 10:581

  2. PubMed ID: 20167119
    Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus
    Skalsky RL, Vanlandingham DL, Scholle F, Higgs S, Cullen BR
    BMC Genomics (2010) 11:119