miRBase entry: hsa-mir-3138

Stem-loop hsa-mir-3138


Accession
MI0014161
Symbol
HGNC: MIR3138
Description
Homo sapiens hsa-mir-3138 precursor miRNA
Gene family
MIPF0001428; mir-3138

Summary
Caution, this is an AI generated summary based on literature. This may have errors. ?

MIR3138 is a microRNA that has been implicated in nerve degeneration and regeneration in diabetic peripheral neuropathy (DPN) [PMC6615525]. The study used pyrosequencing and RT-PCR to validate changes in methylation and gene expression of DPYSL2 and MIR3138 [PMC6615525]. The researchers cross-referenced predicted and known targets of MIR3138 from miRDB and DIANA-TarBase, identifying ERBB4 as a target of MIR3138 [PMC6615525]. ERBB4 has been associated with sensory nerve loss and regeneration, suggesting a potential regulatory role for MIR3138 in DPN [PMC6615525'>PMC6615525'>PMC6615525'>PMC6615525'>PMC6615525]. The study confirmed that differential methylation seen by RRBS (reduced representation bisulfite sequencing) and pyrosequencing was associated with a decrease in MIR3138 gene expression in degenerating sural nerves [PMC6615525]. DPYSL2, NFATC1, and MIR3138 were validated using pyrosequencing and RT-PCR, suggesting that epigenetic downregulation of MIR3138 may play a role in DPN progression [PMC6615525]. The primers for DPYSL2, NFATC1, and MIR3138 were designed at the University of Michigan Epigenomics Core using a published protocol [PMC6615525]. Furthermore, the study found that MIR3138 was the most differentially methylated miRNA between degenerating nerves and regenerating nerves [PMC6615525]. DPYSL2 showed significantly higher methylation levels in the degenerating group compared to the regenerating group by pyrosequencing (p = 0.0005), similar to the results observed for MIR3138 (p = 0.0130) [PMC6615525].

Literature search
4 open access papers mention hsa-mir-3138
(16 sentences)

Sequence

557 reads, 31 reads per million, 53 experiments
cccuccucggcacuucccccaccucacugcccgggugcccacaagacUGUGGACAGUGAGGUAGAGGGAGUgccgaggaggg
(((((((((((((((((.(.((((((((((((((............))).)).))))))))).).)))))))))))))))))

Structure
                 c c         -  -   gugcc 
cccuccucggcacuucc c accucacug cc cgg     c
||||||||||||||||| | ||||||||| || |||      
gggaggagccgUGAGGG G UGGAGUGAC GG GUc     a
                 A A         A  U   agaac 


Annotation confidence Not enough data
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Genome context
chr4: 10078611-10078692 [-]

Database links

Mature hsa-miR-3138

Accession MIMAT0015006
Description Homo sapiens hsa-miR-3138 mature miRNA
Sequence 48 - UGUGGACAGUGAGGUAGAGGGAGU - 71
Evidence experimental
Illumina [1-2]
Database links
Predicted targets

References

  1. PubMed ID: 20300190
    Characterization of the Melanoma miRNAome by Deep Sequencing
    "Stark MS, Tyagi S, Nancarrow DJ, Boyle GM, Cook AL, Whiteman DC, Parsons PG, Schmidt C, Sturm RA, Hayward NK"
    "PLoS One (2010) 5:e9685

  2. PubMed ID: 20224791
    Discovery of novel microRNAs in female reproductive tract using next generation sequencing
    Creighton CJ, Benham AL, Zhu H, Khan MF, Reid JG, Nagaraja AK, Fountain MD, Dziadek O, Han D, Ma L, Kim J, Hawkins SM, Anderson ML, Matzuk MM, Gunaratne PH
    PLoS One (2010) 5:e9637