6 publications mentioning ppe-MIR164c

Open access articles that are associated with the species Prunus persica and mention the gene name MIR164c. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

Analysis of seven drought-responsive miRNAs by qRT-PCR show that the expression level of miR156 and miR168 were high in leaves and roots under drought stress in comparison to control samples while the expression of miR164 and miR395 was down-regulated in root and leaf tissues of drought-stressed samples.

For instance, the decreased expression of miR164 and miR395 promoted the expression of their targets genes in both root and leaf tissues.

Up-regulation of any miRNA expression levels was considered a positive value while negative values indicate down-regulation For miR156, miR164, miR166, miR168, miR169, miR171 and miR395, the miRNA stem-loop reverse transcription reaction was performed in a volume of 10 µL containing 2, 20, and 200 ng of total RNA samples of leaf and root samples (1 µL), 0.5 µL 10 mM dNTP mix, 1 µL stem-loop RT primer (1 µM) and 7.5 µL nuclease free water.

qRT-PCR was also used for detection and quantification of predicted targets of six drougt-responsive miRNAs (miR156, miR164, miR166, miR169, miR171 and miR395).

The relative expression profile of miR156, miR164, miR168, miR171 and miR395 using qRT-PCR had a good correlation with deep sequencing.

Among them, drought responsive miRNAs (miR156, miR164, miR166, miR168, miR169, miR171, and miR395) were detected and their expression levels were measured by qRT-PCR.

2

The ten most highly expressed miRNAs (miR156, miR157, miR159, miR164, miR167, miR172, miR393, miR396, miR414, miR2275, and miR5021) in buds and leaves are miRNAs regulating genes involved in flower and leaf development processes such as integument development, leaf morphogenesis, meristem initiation, maintenance, and growth, bilateral symmetry determination, organ morphogenesis, plant phase transition, shoot apical meristem identity, flower and fruit development, and plant architecture.

Members of four families (miR5021, miR164, miR414, miR396, miR2919) target genes encoding proteins involved in dormancy such as embryo-defective or maternal effect embryo arrest 14 (MEE14).

In silico expression analyses of miRNAs using DEGseq [25] identified 19 sequences belonging to eight conserved miRNA families (miR156, miR157, miR164, miR172, miR393, miR396, miR414, and miR2275) induced in winter buds versus leaves (Additional file 6: Table S6).

Most of conserved families common to Arabidopsis and peach (miR156, miR159, miR160, miR164, miR166, miR171, miR172, miR319, miR390, miR395, and miR396) did not show significant size variation (Figure 4).

3

miR164, miR165, miR166, miR172 and miR393 exhibited a similar phloem-specific expression pattern, i. e., they were highly expressed only in phloem tissues, whereas their expression level was relatively undetectable or very weak in the leaves and fruit at different stages.

miR164, miR166, and miR397 are very similar to the corresponding miRNAs in four plant species, including Brassica napus and Vitis vinifera.

For example, miR156, miR160, miR162, miR164, miR166, miR167, miR169, miR172, miR396, and miR397 are perfectly identical to the corresponding miRNAs in three other plant species (Arabidopsis thaliana, Populus trichocarpa, and Oryza sativa) (S3 Table).

4

miR164 targets CUC1 and CUC2 transcripts in Arabidopsis and controls leaf margin development.

Additionally, for other miRNAs (miR159, miR172, miR164, miR394, and miR160 families), we confirmed miRNA-directed cleavage in one or two Rosa cultivars (Figure  5B-F).

According to previous studies, miR156, miR159, and miR160 are evolutionary conserved in all land plants, and miR164, and miR172 are conserved in seed-bearing plants [57].

5

miR164 is known to target NAC1 to downregulate auxin signal transduction pathway activity [37].

Our analysis also identified miRNA164 cleavage sites in mRNAs of the peach NAC family (Figure 3).

6

In addition, UDP-XYL synthase was a target of miR164.

In addition, 11 miRNA families (miR162, miR164, miR167, miR168, miR169, miR172, miR393, miR394, miR397, miR399 and miR827) shared a high conservation in both dicotyledons and monocotyledons.