6 publications mentioning ppe-MIR390

Open access articles that are associated with the species Prunus persica and mention the gene name MIR390. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

Moreover, TAS3 trans-acting short-interfering RNAs, which are targeted by miR390, can regulate miR166 expression that may control auxin flow via its target HD-ZIP.

The expression levels of miR171, miR168, miR408a, miR398 and miR408b were significantly upregulated in mesocarp in NAA -treated samples compared to the control fruits, whereas those of miR156, miR160, miR166, miR167, miR390, miR393, miR482, miR535 and miR2118 were downregulated following NAA treatment.

miR390 can indirectly inhibit the expression of ARF2, ARF3 and ARF4 in Arabidopsis by targeting TAS3-derived trans-acting short-interfering RNAs (tasiRNAs) [18].

In our study, miR390 expression levels were strongly reduced in the NAA -treated fruit, indicating that this miRNA may regulate fruit development by controlling expression of ARFs.

The results of real-time PCR experiments revealed the increased expression levels of miR171, miR168, miR408a, miR398 and miR408b, as well as the reduced expression levels of miR166, miR167, miR160, miR156, miR2118, miR535, miR390, miR482 and miR393 in the peach fruit after NAA treatment, respectively, suggesting the functional divergence of microRNAs in the regulation of fruit development.

miR390 was previously shown to repress the expression of ARFs by regulating tasiRNAs expression profiles [18].

The experimental verification results were consistent with our high throughput sequencing datasets and also with previous studies that demonstrated the critical roles of miR160, miR166, miR167, miR390 and miR393 and their targeted genes in auxin signaling pathways.

Among these miRNAs, miR160, miR166, miR167, miR390 and miR393 are known to play important roles in auxin signaling pathways [14, 16, 17, 18, 35, 36].

2

MiR390's cleavage target, a TAS3 ortholog (EST: AJ875750, defined as PpTAS3) was identified in peach, and shared similar dual miR390 target sites with its Arabidopsis counterpart.

Since auxin signaling and modulation is essential for diverse biological processes in peach, especially for fruit development and ripening [66, 67], miR390- TAS3 biogenesis-derived tasiARFs in specific tissues could orchestrate auxin signaling that could be directly relevant to fruit growth and development.

showed that both miR390 and miR828 had detectable expression in various peach tissues (Figure 2b).

Mostly 21-nt tasiRNAs were generated in the flower tissue (Figure 4a), which correlated with flower-specific expression of miR390 (Figure 2b).

The tissue-specific expression patterns were presented for the conserved miRNAs, miR160, miR167, miR169, miR319, miR390 and miR396, and the less-conserved miRNAs, miR828, miR858 and miR2118 (Figure 2b).

Transcript precursors were observed for miR390 by RT-PCR in the L1 but not the L2 layer of the shoot apical meristem while similar levels of mature miR390 was revealed in the same L1 and L2 layers by in situ hybridization [56].

These results suggest that both miR166a and miR390 precursors could be differentially processed in various cell types or tissues, although other possibilities, including miRNA stability and mobility, could not be ruled out [56, 57].

org/node/35) or the EST sequence in the case of miR390.

In this study, we found that both miR390- TAS3 and miR828- TAS4 tasiRNA pathways are conserved in peach as evidenced by the identification of miR390 and miR828, TAS3 and TAS4 transcripts and the generation of phased 21-nt siRNAs along both TAS3 and TAS4 transcripts (Figure 4a,b).

Further, both miR390- TAS3 and miR828- TAS4 siRNA biogenesis pathways and their functions appear to be conserved in peach; miR828 cleavage is capable of activating siRNA biogenesis in PpTAS4 and three MYB protein-coding transcripts, indicating a silencing reinforcement in peach.

Trans-acting siRNAs in peachIn this study, we found that both miR390- TAS3 and miR828- TAS4 tasiRNA pathways are conserved in peach as evidenced by the identification of miR390 and miR828, TAS3 and TAS4 transcripts and the generation of phased 21-nt siRNAs along both TAS3 and TAS4 transcripts (Figure 4a,b).

Together, these data indicate that both miR390- TAS3 and miR828- TAS4 biogenesis pathways and functions are at least partially conserved in peach.

3

The miR160 were solely up-regulated in root, whereas there were no changes in the expression level of miR168, miR390 in leaf and root tissues (Table 5).

4

miR156, miR159, miR166, miR172, miR390, miR396, and miR5021 are the most expressed families in bud tissues.

Most of conserved families common to Arabidopsis and peach (miR156, miR159, miR160, miR164, miR166, miR171, miR172, miR319, miR390, miR395, and miR396) did not show significant size variation (Figure 4).

5

Among the miRNA families in peach, miR156, miR159, miR160, miR166, miR171, miR319, miR390 and miR396 showed a high conservation in plants, indicating that these 12 peach miRNA families are ancient.

6

In the aging pathway, it has been found that the role of five microRNAs (miRNAs) families called miR156, miR172, miR159/319, miR390, and miR399 is important in flowering time (Spanudakis and Jackson, 2014).