miRBase entry: hsa-mir-21

Stem-loop hsa-mir-21

Homo sapiens hsa-mir-21 precursor miRNA
Gene family
MIPF0000060; mir-21

Caution, this is an AI generated summary based on literature. This may have errors. ?

To examine the signaling pathways involved in MIR21 mimic-induced wound healing responses, western blot analysis was performed on wounded tissues on day 5 [PMC7053209]. Serum MIR21, CEA, NSE, and CYFRA21-1 levels were, on average, significantly higher in patients with NSCLC than in control individuals (Table 1) [PMC4737022]. However, serum MIR21 and CYFRA21-1 levels of NSCLC patients at TNM stages I-II were significantly lower than those of patients at TNM stages III-IV (t = 2.575, −2.301, resp [PMC4737022]. Comparison of joint detection of serum CYFRA21-1 and MIR21 levels with single detection of either marker indicated that AUC of single detection for CYFRA21-1, single detection for MIR21, and joint detection of both markers were 0.843, 0.872, and 0.909, respectively (Figure 1(c), Table 6) [PMC4737022]. Therefore, this study comparatively analyzed the value of MIR21 compared to tumor markers CEA, NSE, and CYFRA21-1 for early diagnosis of NSCLC [PMC4737022'>PMC4737022'>PMC4737022'>PMC4737022'>PMC4737022'>PMC4737022'>PMC4737022'>PMC4737022'>PMC4737022]. However, logistic multiple regression analysis indicated that early NSCLC (TNM stages I-II) was associated with serum CYFRA21-1 and MIR21 levels [PMC4737022]. Joint detection of MIR21 with serum CYFRA21-1 can further improve diagnostic efficiency for early NSCLC [PMC4737022]. The results indicated that occurrence of early NSCLC was significantly correlated with serum CYFRA21-1 (OR = 1.076; Wald χ 2 = 4.025) and MIR21 (OR = 2.473; Wald χ 2 = 9.153) levels (P < 0.05) (Table 5) [PMC4737022]. MIR21 had the highest diagnostic efficiency for early NSCLC (Figure 1(b), Table 4) [PMC4737022]. Therefore, MIR21 could serve as an important serum marker for auxiliary diagnosis of early NSCLC, while joint detection of serum MIR21 and CYFRA21-1 levels could improve diagnostic efficiency [PMC4737022]. This study indicates that, on average, serum MIR21 and CYFRA21-1 levels were lower in patients with NSCLC at TNM stages I-II than NSCLC at TNM stages III-IV [PMC4737022]. Serum MIR21, CEA, NSE, and CYFRA21-1 levels did not significantly differ with gender or age of NSCLC patients or with tumor pathology (Table 2) [PMC4737022]. AUC analysis showed that MIR21 had the highest diagnostic efficiency for early NSCLC [PMC4737022]. After removing the data for TNM stages III-IV, the AUC of using serum MIR21, CEA, NSE, and CYFRA21-1 levels to diagnose early NSCLC (TNM stages I-II) was 0.752, 0.806, 0.843, and 0.882, respectively [PMC4737022]. To validate the relationship between MIR21 expression and AUY922 sensitivity, we conducted a high-throughput compound screen in RKO cells that had been engineered to knock out the MIR21 locus (MIR21KO) and parental isogenic wild type (WT) cells.40 A number of HSP90 inhibitors produced a larger reduction in cell viability in MIR21KO cells in comparison with WT cells (Supplementary Figure 2A), with AUY922, 17-AAG, 17-DMAG, and ganetespib showing the highest activity [PMC5863695]. To validate the role of MIR21 in driving resistance to HSP90 inhibition, we infected MIR21KO DLD-1 cells with an inducible MIR21 or control (CTRL) viral vector (Supplementary Figure 2F) and monitored their response to AUY922 [PMC5863695]. A multiplex sandwich immunoassay showed a reduction in the level of HSP40 (

Literature search
1559 open access papers mention hsa-mir-21
(13078 sentences)


6832853 reads, 28570 reads per million, 159 experiments

                A     A     A    u  a 
ugucgggUAGCUUAUC GACUG UGUUG cugu ga  
|||||||||||||||| ||||| ||||| |||| || u
                -     C     -    -  c 

Annotation confidence High
Do you think this miRNA is real?
Mourelatos et al. named this sequence miR-21 precursor-17 and also reported the exact reverse complement of this predicted stem-loop sequence and erroneously assigned the name miR-104 [2].

Genome context
chr17: 59841266-59841337 [+]

Disease association
hsa-mir-21 is associated with one or more human diseases in the Human microRNA Disease Database
Disease Description Category PubMed ID

Biological pathways
hsa-mir-21 is involved in one or more biological pathways:
(Source: Reactome)
Biological reactions
hsa-mir-21 is involved in one or more regulation/signalling events:
(Source: Reactome)

Database links

Mature hsa-miR-21-5p

Accession MIMAT0000076
Description Homo sapiens hsa-miR-21-5p mature miRNA
Evidence experimental
cloned [1-3,6-9], Northern [1,5], Illumina [10]
Database links
Predicted targets

Mature hsa-miR-21-3p

Accession MIMAT0004494
Description Homo sapiens hsa-miR-21-3p mature miRNA
Evidence experimental
cloned [8-9]
Database links
Predicted targets


  1. PubMed ID: 11679670
    Identification of novel genes coding for small expressed RNAs
    "Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T"
    "Science (2001) 294:853-858

  2. PubMed ID: 15183728
    Human embryonic stem cells express a unique set of microRNAs
    "Suh MR, Lee Y, Kim JY, Kim SK, Moon SH, Lee JY, Cha KY, Chung HM, Yoon HS, Moon SY, Kim VN, Kim KS"
    "Dev Biol (2004) 270:488-498

  3. PubMed ID: 12554860
    Numerous microRNPs in neuronal cells containing novel microRNAs
    "Dostie J, Mourelatos Z, Yang M, Sharma A, Dreyfuss G"
    "RNA (2003) 9:180-186

  4. PubMed ID: 14573789
    Reduced accumulation of specific microRNAs in colorectal neoplasia
    "Michael MZ, O' Connor SM, van Holst Pellekaan NG, Young GP, James RJ"
    "Mol Cancer Res (2003) 1:882-891

  5. PubMed ID: 15325244
    Altered expression profiles of microRNAs during TPA-induced differentiation of HL-60 cells
    "Kasashima K, Nakamura Y, Kozu T"
    "Biochem Biophys Res Commun (2004) 322:403-410

  6. PubMed ID: 17604727
    A mammalian microRNA expression atlas based on small RNA library sequencing
    "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
    "Cell (2007) 129:1401-1414

  7. PubMed ID: 17616659
    Patterns of known and novel small RNAs in human cervical cancer
    "Lui WO, Pourmand N, Patterson BK, Fire A"
    "Cancer Res (2007) 67:6031-6043

  8. PubMed ID: 20158877
    Analysis of deep sequencing microRNA expression profile from human embryonic stem cells derived mesenchymal stem cells reveals possible role of let-7 microRNA family in downstream targeting of hepatic nuclear factor 4 alpha
    Koh W, Sheng CT, Tan B, Lee QY, Kuznetsov V, Kiang LS, Tanavde V
    BMC Genomics (2010) 11:S6

  9. PubMed ID: 11914277
    miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs
    "Mourelatos Z, Dostie J, Paushkin S, Sharma A, Charroux B, Abel L, Rappsilber J, Mann M, Dreyfuss G"
    "Genes Dev (2002) 16:720-728

  10. PubMed ID: 15978578
    Identification of human fetal liver miRNAs by a novel method
    "Fu H, Tie Y, Xu C, Zhang Z, Zhu J, Shi Y, Jiang H, Sun Z, Zheng X"
    "FEBS Lett (2005) 579:3849-3854