miRBase entry: cel-mir-250

Stem-loop cel-mir-250


Accession
MI0000326
Description
Caenorhabditis elegans cel-mir-250 precursor miRNA mir-250
Gene
family?
RF00836; mir-250

Literature search
1 open access papers mention cel-mir-250
(1 sentences)

Sequence

389836 reads, 3282 reads per million, 16 experiments
agggucuucggaccacgCCUUCAGUUGCCUCGUGAUCCGccaaacacaauaaauggacgAAUCACAGUCAACUGUUGGCauggugcucguacccauuuu
.((((....(.((((.(((..((((((.((.(((((.(((((...........))).)).))))))).))))))..))).)))).)....)))).....

Structure
----a    cuuc g    c   UU      C  C     C  -   aaca 
     gggu    g acca gCC  CAGUUG CU GUGAU CG cca    c
     ||||    | |||| |||  |||||| || ||||| || |||    a
     ccca    c uggu CGG  GUCAAC GA CACUA gc ggu    a
uuuua    ugcu g    a   UU      U  -     A  a   aaau 


Annotation confidence High
Do you think this miRNA is real?
Comments
The extents of the dominant mature miRNA species are adjusted here in accordance with a large scale cloning and sequencing study [2].

Genome context
chrV: 11769917-11770015 [-]
Clustered miRNAs
1 other miRNA is < 10 kb from cel-mir-250
Name Accession Chromosome Start End Strand Confidence




Database links

Mature cel-miR-250-3p

Accession MIMAT0000306
Description Caenorhabditis elegans cel-miR-250-3p mature miRNA
Sequence 60 - AAUCACAGUCAACUGUUGGC - 79
Evidence experimental
Northern [1-2], PCR [1], Illumina [3,5], CLIPseq [4]
Database links
Predicted targets

Mature cel-miR-250-5p

Accession MIMAT0020339
Description Caenorhabditis elegans cel-miR-250-5p mature miRNA
Sequence 18 - CCUUCAGUUGCCUCGUGAUCCG - 39
Evidence experimental
Illumina [5]
Database links

References

  1. PubMed ID: 12672692
    The microRNAs of Caenorhabditis elegans
    "Lim LP, Lau NC, Weinstein EG, Abdelhakim A, Yekta S, Rhoades MW, Burge CB, Bartel DP"
    "Genes Dev (2003) 17:991-1008

  2. PubMed ID: 17174894
    Large-scale sequencing reveals 21U-RNAs and additional microRNAs and endogenous siRNAs in C. elegans
    "Ruby JG, Jan C, Player C, Axtell MJ, Lee W, Nusbaum C, Ge H, Bartel DP"
    "Cell (2006) 127:1193-1207

  3. PubMed ID: 19460142
    Dynamic expression of small non-coding RNAs, including novel microRNAs and piRNAs/21U-RNAs, during Caenorhabditis elegans development
    Kato M, de Lencastre A, Pincus Z, Slack FJ
    Genome Biol (2009) 10:R54

  4. PubMed ID: 20062054
    Comprehensive discovery of endogenous Argonaute binding sites in Caenorhabditis elegans
    "Zisoulis DG, Lovci MT, Wilbert ML, Hutt KR, Liang TY, Pasquinelli AE, Yeo GW"
    "Nat Struct Mol Biol (2010) 17:173-179

  5. PubMed ID: 21307183
    Improved annotation of C. elegans microRNAs by deep sequencing reveals structures associated with processing by Drosha and Dicer
    "Warf MB, Johnson WE, Bass BL"
    "RNA (2011) 17:563-577