miRBase entry: dme-mir-305

Stem-loop dme-mir-305


Accession
MI0000412
Description
Drosophila melanogaster dme-mir-305 precursor miRNA mir-305
Gene
family?
RF00732; mir-305

Literature search
12 open access papers mention dme-mir-305
(34 sentences)

Sequence

663514 reads, 12557 reads per million, 49 experiments
caugucuAUUGUACUUCAUCAGGUGCUCUGgugugucucguaaccCGGCACAUGUUGAAGUACACUCAauaug
(((((....(((((((((.((.(((((..((.((........))))))))).)).)))))))))....)))))

Structure
     cuAU         U  G     CU  u  guc 
caugu    UGUACUUCA CA GUGCU  Gg gu   u
|||||    ||||||||| || |||||  || ||    
guaua    ACAUGAAGU GU CACGG  Cc ca   c
     ACUC         U  A     --  -  aug 


Annotation confidence High
Do you think this miRNA is real?
Comments
Lai et al. predicted this sequence based on conservation in D. pseudoobscura, but did not verify expression [2]. Aravin et al. describe identification and mapping of the 5' end of the excised sequence by cloning [1].

Genome context
chr2L: 7425972-7426044 [+]
Clustered miRNAs
1 other miRNA is < 10 kb from dme-mir-305
Name Accession Chromosome Start End Strand Confidence




Database links

Mature dme-miR-305-5p

Accession MIMAT0000391
Description Drosophila melanogaster dme-miR-305-5p mature miRNA
Sequence 8 - AUUGUACUUCAUCAGGUGCUCUG - 30
Evidence experimental
cloned [1], 454 [3-4], Illumina [4]
Database links
Predicted targets

Mature dme-miR-305-3p

Accession MIMAT0020827
Description Drosophila melanogaster dme-miR-305-3p mature miRNA
Sequence 46 - CGGCACAUGUUGAAGUACACUCA - 68
Evidence not_experimental
Database links

References

  1. PubMed ID: 12919683
    The small RNA profile during Drosophila melanogaster development
    "Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T"
    "Dev Cell (2003) 5:337-350

  2. PubMed ID: 17989254
    Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
    "Genome Res (2007) 17:1850-1864

  3. PubMed ID: 17989255
    Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
    "Genome Res (2007) 17:1865-1879

  4. PubMed ID: 12844358
    Computational identification of Drosophila microRNA genes
    "Lai EC, Tomancak P, Williams RW, Rubin GM"
    "Genome Biol (2003) 4:R42