Stem-loop mmu-mir-485

Accession

MI0003492

Symbol

MGI: Mir485

Description

Mus musculus mmu-mir-485 precursor miRNA mir-485

Gene
family

RF04296; mir-485

Literature search

147 open access papers mention mmu-mir-485
(367 sentences)

Sequence

54661 reads, 223.0 reads per million, 140 experiments

acuuggagAGAGGCUGGCCGUGAUGAAUUCGauucaucuaaacgaGUCAUACACGGCUCUCCUCUCUUCuagu
(((.(((((((((..(((((((((((.((((...........)))))))).)))))))..))))))))).)))

Structure

   u         CU       -    A    auuc 
acu ggagAGAGG  GGCCGUG AUGA UUCG    a
||| |||||||||  ||||||| |||| ||||    u
uga CUUCUCUCC  UCGGCAC UACU Gagc    c
   u         UC       A    -    aaau 

Annotation confidence High

Comments

miR-485-3p was predicted by Sewer et al [1], and its expression later verified experimentally by Mineno et al using MPSS technology [2]. The MPSS protocol used provides 22nt sequences, but the true extents of the mature miRNA are not reliably obtained. Landgraf et al. show that the 5' product is the predominant one [3].

Genome context

12: 109701336-109701408 [+]

Clustered miRNAs

16 other miRNAs are < 10 kb from mmu-mir-485

Mature mmu-miR-485-5p

• Accession: MIMAT0003128
• Description: Mus musculus mmu-miR-485-5p mature miRNA
• Sequence: 9 - AGAGGCUGGCCGUGAUGAAUUCG - 31
• Evidence: experimental ; cloned [1,3], MPSS [2], Illumina [4-5]

Mature mmu-miR-485-3p

• Accession: MIMAT0003129
• Description: Mus musculus mmu-miR-485-3p mature miRNA
• Sequence: 46 - GUCAUACACGGCUCUCCUCUCUUC - 69
• Evidence: experimental ; MPSS [2], Illumina [4-5]

References

1. PubMed ID: 17604727
   A mammalian microRNA expression atlas based on small RNA library sequencing
   "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
   "Cell (2007) 129:1401-1414


2. PubMed ID: 20215419
   MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing
   "Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A"
   "Mol Hum Reprod (2010) 16:463-471


3. PubMed ID: 20413612
   Mammalian microRNAs: experimental evaluation of novel and previously annotated genes
   "Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP"
   "Genes Dev (2010) 24:992-1009


4. PubMed ID: 16582102
   The expression profile of microRNAs in mouse embryos
   "Mineno J, Okamoto S, Ando T, Sato M, Chono H, Izu H, Takayama M, Asada K, Mirochnitchenko O, Inouye M, Kato I"
   "Nucleic Acids Res (2006) 34:1765-1771


5. PubMed ID: 16274478
   Identification of clustered microRNAs using an ab initio prediction method
   Sewer A, Paul N, Landgraf P, Aravin A, Pfeffer S, Brownstein MJ, Tuschl T, van Nimwegen E, Zavolan M
   BMC Bioinformatics (2005) 6:267