Stem-loop mmu-mir-547

Accession

MI0003523

Symbol

MGI: Mir547

Description

Mus musculus mmu-mir-547 precursor miRNA mir-547

Gene
family

RF03503; mir-547

Literature search

4 open access papers mention mmu-mir-547
(5 sentences)

Sequence

3547 reads, 281 reads per million, 49 experiments

gugugauguauCACUUGAGGAUGUACCACCCAuuuaacaggaaacaugCUUGGUACAUCUUUGAGUGAGauaacacac
(((((.(((.((((((((((((((((((..(((............)))..)))))))))))))))))).))).)))))

Structure

     a   a                  CC   uuaac 
gugug ugu uCACUUGAGGAUGUACCA  CAu     a
||||| ||| ||||||||||||||||||  |||      
cacac aua AGUGAGUUUCUACAUGGU  gua     g
     a   G                  UC   caaag 

Annotation confidence High

Comments

The predominant miRNA cloned by Langraf et al. has a 3' terminal U residue, which is incompatible with the genome sequence [2].

Genome context

chrX: 67988374-67988451 [-]

Clustered miRNAs

1 other miRNA is < 10 kb from mmu-mir-547

Mature mmu-miR-547-5p

• Accession: MIMAT0017210
• Description: Mus musculus mmu-miR-547-5p mature miRNA
• Sequence: 12 - CACUUGAGGAUGUACCACCCA - 32
• Evidence: experimental ; Illumina [4]

Mature mmu-miR-547-3p

• Accession: MIMAT0003173
• Description: Mus musculus mmu-miR-547-3p mature miRNA
• Sequence: 49 - CUUGGUACAUCUUUGAGUGAG - 69
• Evidence: experimental ; cloned [1-2], Illumina [3-4]

References

1. PubMed ID: 17604727
   A mammalian microRNA expression atlas based on small RNA library sequencing
   "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
   "Cell (2007) 129:1401-1414


2. PubMed ID: 20215419
   MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing
   "Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A"
   "Mol Hum Reprod (2010) 16:463-471


3. PubMed ID: 20413612
   Mammalian microRNAs: experimental evaluation of novel and previously annotated genes
   "Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP"
   "Genes Dev (2010) 24:992-1009


4. PubMed ID: 16274478
   Identification of clustered microRNAs using an ab initio prediction method
   Sewer A, Paul N, Landgraf P, Aravin A, Pfeffer S, Brownstein MJ, Tuschl T, van Nimwegen E, Zavolan M
   BMC Bioinformatics (2005) 6:267