Stem-loop ppe-MIR482e

Accession

MI0021655

Description

Prunus persica ppe-MIR482e precursor miRNA

Literature search

2 open access papers mention ppe-MIR482e
(6 sentences)

Sequence

185310 reads, 3927.0 reads per million, 20 experiments

gaggaaguuuuuGGCCAUGGGAAGUUUGGCAAGAaauauuuauuauauauucuacuacuucaaguucuguuugaaguauguauguauaaguaguucUUGCCUAUUCCUCCCAUGCCAAugauuuccua
.((((((((.(((((.((((((.(...((((((((.(((((((((((((......(((((((((.....))))))))))))))).))))))).))))))))....).))))))))))).)))))))).

Structure

g        u     C      A -UUU        a       -      uucuac         u 
 aggaaguu uuGGC AUGGGA G    GGCAAGAa uauuuau uauaua      uacuucaag u
 |||||||| ||||| |||||| |    |||||||| ||||||| ||||||      ||||||||| c
 uccuuuag AACCG UACCCU C    CCGUUcuu augaaua guaugu      augaaguuu u
a        u     -      C UUAU        g       u      ------         g 

Annotation confidence High

Comments

Gao et al. identified small RNAs by sequencing in Prunus mume [1]. The reads were mapped to the Prunus persica genome. This entry represents the Prunus persica sequence, but the evidence for mature RNA expression is from Prunus mume.

Genome context

chrG1: 30620690-30620817 [+]

Clustered miRNAs

3 other miRNAs are < 10 kb from ppe-MIR482e

Mature ppe-miR482e-5p

• Accession: MIMAT0058485
• Description: Prunus persica ppe-miR482e-5p mature miRNA
• Sequence: 13 - GGCCAUGGGAAGUUUGGCAAGA - 34
• Evidence: experimental ; unknown

Mature ppe-miR482e-3p

• Accession: MIMAT0027318
• Description: Prunus persica ppe-miR482e-3p mature miRNA
• Sequence: 97 - UUGCCUAUUCCUCCCAUGCCAA - 118
• Evidence: experimental ; Illumina [1]

References

1. PubMed ID: 22863067
   High-throughput sequencing of small RNAs and analysis of differentially expressed microRNAs associated with pistil development in Japanese apricot
   Gao Z, Shi T, Luo X, Zhang Z, Zhuang W, Wang L
   BMC Genomics (2012) 13:371