miRBase entry: hsa-mir-196a-1

Stem-loop hsa-mir-196a-1


Accession
MI0000238
Symbol
HGNC: MIR196A1
Description
Homo sapiens hsa-mir-196a-1 precursor miRNA
Gene family
MIPF0000031; mir-196

Summary
Caution, this is an AI generated summary based on literature. This may have errors. ?

MIR196A1 is a member of the MIR196 gene family, which includes MIR196A1, MIR196A2, and MIR196B. It is a noncoding RNA derived from the HOXB gene cluster on chromosome 17 in humans. [PMC4413621]. Studies have identified MIR196A1 as a potential biomarker for several diseases, including hepatocellular carcinoma, leukemia, lung cancer, breast cancer, colorectal cancer, and pancreatic adenocarcinoma [PMC10134363]. The expression of MIR196A1 can be influenced by genetic variations and is correlated with HOXB7 and HOXB8 expression [PMC5551414]. It has also been associated with adipogenesis and body fat distribution [PMC5551414]. The miR-196 family includes miR-196a-2 (encoded by MIR196A2) and miR-196b (encoded by MIR196B), which share identical seed sequences with miR-196a1 [PMC6080909]. Aberrant methylation of the CpG sites in the RUNX3 and MIR196A1 genes has been observed in relation to smoking cessation [PMC5960087]. Additionally, the combination of VAT1L, CALR, LINC01456, RP11-484L8.1, MIR148A, and MIR 19 6 A 1 has been associated with overall survival in pancreatic cancer patients [PMC5641173]. Overall,MIR19 6 A 1 plays a role in various diseases as a potential biomarker or regulator of gene expression.

Literature search
215 open access papers mention hsa-mir-196a-1
(1246 sentences)

Sequence

150959 reads, 1109 reads per million, 95 experiments
gugaauUAGGUAGUUUCAUGUUGUUGGGccuggguuucugaacaCAACAACAUUAAACCACCCGAuucac
(((((((.(((.((((.((((((((((..(.........)..).))))))))).)))).))).)))))))

Structure
       A   A    C         - Gc ugg 
gugaauU GGU GUUU AUGUUGUUG G  c   g
||||||| ||| |||| ||||||||| |  |   u
cacuuAG CCA CAAA UACAACAAC c  g   u
       C   C    U         a aa ucu 


Annotation confidence High
Do you think this miRNA is real?
Comments
Lagos-Quintana et al. [1] cloned this miRNA from human osteoblast sarcoma cells. Lim et al [2] independently predicted this miRNA by computational methods using conservation with mouse and Fugu rubripes sequences [2]. Expression of the excised miR was validated in zebrafish. The sequence maps to human chromosome 17. Yekta et al. report that miR-196 miRNAs are expressed from HOX gene clusters in mammals, and that HOX genes in these clusters are targets of miR-196. Indeed, HOXB8 mRNA was shown to be a natural target for miR-196-directed cleavage through a perfectly complementary miR-target site. Other HOX genes have imperfect miR-196 complementary sites indicative of regulation by translational repression [3]. The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [4].

Genome context
chr17: 48632490-48632559 [-]

Disease association
hsa-mir-196a-1 is associated with one or more human diseases in the Human microRNA Disease Database
Disease Description Category PubMed ID

Biological pathways
hsa-mir-196a-1 is involved in one or more biological pathways:
(Source: Reactome)
Biological reactions
hsa-mir-196a-1 is involved in one or more regulation/signalling events:
(Source: Reactome)

Database links

Mature hsa-miR-196a-5p

Accession MIMAT0000226
Description Homo sapiens hsa-miR-196a-5p mature miRNA
Sequence 7 - UAGGUAGUUUCAUGUUGUUGGG - 28
Evidence experimental
cloned [3-5]
Database links
Predicted targets

Mature hsa-miR-196a-1-3p

Accession MIMAT0037307
Description Homo sapiens hsa-miR-196a-1-3p mature miRNA
Sequence 45 - CAACAACAUUAAACCACCCGA - 65
Evidence not_experimental

References

  1. PubMed ID: 17604727
    A mammalian microRNA expression atlas based on small RNA library sequencing
    "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
    "Cell (2007) 129:1401-1414

  2. PubMed ID: 17616659
    Patterns of known and novel small RNAs in human cervical cancer
    "Lui WO, Pourmand N, Patterson BK, Fire A"
    "Cancer Res (2007) 67:6031-6043

  3. PubMed ID: 12624257
    Vertebrate microRNA genes
    "Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP"
    "Science (2003) 299:1540

  4. PubMed ID: 12554859
    New microRNAs from mouse and human
    "Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T"
    "RNA (2003) 9:175-179

  5. PubMed ID: 15105502
    MicroRNA-directed cleavage of HOXB8 mRNA
    "Yekta S, Shih IH, Bartel DP"
    "Science (2004) 304:594-596