Stem-loop hsa-mir-196a-2

Accession

MI0000279

Symbol

HGNC: MIR196A2

Description

Homo sapiens hsa-mir-196a-2 precursor miRNA

Literature search

227 open access papers mention hsa-mir-196a-2
(1268 sentences)

Sequence

156910 reads, 712 reads per million, 95 experiments

ugcucgcucagcugaucuguggcuUAGGUAGUUUCAUGUUGUUGGGauugaguuuugaacuCGGCAACAAGAAACUGCCUGAGuuacaucagucgguuuucgucgagggc
..(((((..(((((((.((((((((((((((((((.((((((((((.((........)))))))))))).))))))))))))))))))...)))))))...).))))...

Structure

-ug    - -uc       --c                  A          a  gag 
   cucg c   agcugau   uguggcuUAGGUAGUUUC UGUUGUUGGG uu   u
   |||| |   |||||||   |||||||||||||||||| |||||||||| ||    
   gagc g   uuggcug   acauuGAGUCCGUCAAAG ACAACGGCuc aa   u
cgg    u cuu       acu                  A          -  guu 

Annotation confidence High

Comments

This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences [1]. Expression of the excised miR has been validated in zebrafish, and the ends mapped by cloning. miR-196a was later validated in human [3,4]. Yekta et al. report that miR-196 miRNAs are expressed from HOX gene clusters in mammals, and that HOX genes in these clusters are targets of miR-196. Indeed, HOXB8 mRNA was shown to be a natural target for miR-196-directed cleavage through a perfectly complementary miR-target site. Other HOX genes have imperfect miR-196 complementary sites indicative of regulation by translational repression [2]. The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [4].

Genome context

chr12: 53991738-53991847 [+]

Disease association

hsa-mir-196a-2 is associated with one or more human diseases in the Human microRNA Disease Database

Mature hsa-miR-196a-5p

• Accession: MIMAT0000226
• Description: Homo sapiens hsa-miR-196a-5p mature miRNA
• Sequence: 25 - UAGGUAGUUUCAUGUUGUUGGG - 46
• Evidence: experimental ; cloned [3-5]

Mature hsa-miR-196a-3p

• Accession: MIMAT0004562
• Description: Homo sapiens hsa-miR-196a-3p mature miRNA
• Sequence: 62 - CGGCAACAAGAAACUGCCUGAG - 83
• Evidence: experimental ; cloned [4]

References

1. PubMed ID: 17604727
   A mammalian microRNA expression atlas based on small RNA library sequencing
   "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
   "Cell (2007) 129:1401-1414


2. PubMed ID: 17616659
   Patterns of known and novel small RNAs in human cervical cancer
   "Lui WO, Pourmand N, Patterson BK, Fire A"
   "Cancer Res (2007) 67:6031-6043


3. PubMed ID: 12624257
   Vertebrate microRNA genes
   "Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP"
   "Science (2003) 299:1540


4. PubMed ID: 12554859
   New microRNAs from mouse and human
   "Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T"
   "RNA (2003) 9:175-179


5. PubMed ID: 15105502
   MicroRNA-directed cleavage of HOXB8 mRNA
   "Yekta S, Shih IH, Bartel DP"
   "Science (2004) 304:594-596