miRBase entry: dme-mir-184

Stem-loop dme-mir-184


Accession
MI0000354
Description
Drosophila melanogaster dme-mir-184 precursor miRNA
Gene family
MIPF0000059; mir-184

Literature search
28 open access papers mention dme-mir-184
(58 sentences)

Sequence

21531115 reads, 120037 reads per million, 49 experiments
gguuggccggugcauucguacCCUUAUCAUUCUCUCGCCCCGugugcacuuaaagacaacUGGACGGAGAACUGAUAAGGGCucguaucaccaauucauc
((....))((((.((.((..((((((((((((((.((((...(((..........)))...)).))))))).)))))))))..)).))))))........

Structure
gguuggcc    c  u  ua         -     U  -  CCG   gcac 
        ggug au cg  cCCUUAUCA UUCUC CG CC   ugu    u
        |||| || ||  ||||||||| ||||| || ||   |||     
        ccac ua gc  GGGAAUAGU AAGAG GC GG   aca    u
cuacuuaa    -  u  uC         C     -  A  Uca   gaaa 


Annotation confidence High
Do you think this miRNA is real?
Comments
miR-184 was reported independently in references [1] and [2]. Computational prediction followed by northern blotting confirmed that the strand containing the predicted miR is predominantly expressed [1]. Reference [2] confirmed the 5' end of the excised miRNA by cloning and reported a length distribution of 19-23 nt with 22 nt the most commonly expressed. They also reported the less predominantly expressed sequence miR-184* from the opposite 5' arm.

Genome context
chr2R: 13329402-13329501 [-]

Database links

Mature dme-miR-184-5p

Accession MIMAT0000330
Description Drosophila melanogaster dme-miR-184-5p mature miRNA
Sequence 22 - CCUUAUCAUUCUCUCGCCCCG - 42
Evidence experimental
cloned [2], 454 [3-4], Illumina [4]
Database links

Mature dme-miR-184-3p

Accession MIMAT0000331
Description Drosophila melanogaster dme-miR-184-3p mature miRNA
Sequence 61 - UGGACGGAGAACUGAUAAGGGC - 82
Evidence experimental
Northern [1], cloned [2], 454 [3-4], Illumina [4]
Database links
Predicted targets

References

  1. PubMed ID: 12919683
    The small RNA profile during Drosophila melanogaster development
    "Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T"
    "Dev Cell (2003) 5:337-350

  2. PubMed ID: 17989254
    Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
    "Genome Res (2007) 17:1850-1864

  3. PubMed ID: 17989255
    Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
    "Genome Res (2007) 17:1865-1879

  4. PubMed ID: 12844358
    Computational identification of Drosophila microRNA genes
    "Lai EC, Tomancak P, Williams RW, Rubin GM"
    "Genome Biol (2003) 4:R42