Stem-loop dme-mir-184

Accession

MI0000354

Description

Drosophila melanogaster dme-mir-184 precursor miRNA

Literature search

28 open access papers mention dme-mir-184
(58 sentences)

Sequence

21531115 reads, 119755 reads per million, 49 experiments

gguuggccggugcauucguacCCUUAUCAUUCUCUCGCCCCGugugcacuuaaagacaacUGGACGGAGAACUGAUAAGGGCucguaucaccaauucauc
((....))((((.((.((..((((((((((((((.((((...(((..........)))...)).))))))).)))))))))..)).))))))........

Structure

gguuggcc    c  u  ua         -     U  -  CCG   gcac 
        ggug au cg  cCCUUAUCA UUCUC CG CC   ugu    u
        |||| || ||  ||||||||| ||||| || ||   |||     
        ccac ua gc  GGGAAUAGU AAGAG GC GG   aca    u
cuacuuaa    -  u  uC         C     -  A  Uca   gaaa 

Annotation confidence Medium

Comments

miR-184 was reported independently in references [1] and [2]. Computational prediction followed by northern blotting confirmed that the strand containing the predicted miR is predominantly expressed [1]. Reference [2] confirmed the 5' end of the excised miRNA by cloning and reported a length distribution of 19-23 nt with 22 nt the most commonly expressed. They also reported the less predominantly expressed sequence miR-184* from the opposite 5' arm.

Genome context

chr2R: 13329402-13329501 [-]

Mature dme-miR-184-5p

• Accession: MIMAT0000330
• Description: Drosophila melanogaster dme-miR-184-5p mature miRNA
• Sequence: 22 - CCUUAUCAUUCUCUCGCCCCG - 42
• Evidence: experimental ; cloned [2], 454 [3-4], Illumina [4]

Mature dme-miR-184-3p

• Accession: MIMAT0000331
• Description: Drosophila melanogaster dme-miR-184-3p mature miRNA
• Sequence: 61 - UGGACGGAGAACUGAUAAGGGC - 82
• Evidence: experimental ; Northern [1], cloned [2], 454 [3-4], Illumina [4]

References

1. PubMed ID: 12919683
   The small RNA profile during Drosophila melanogaster development
   "Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T"
   "Dev Cell (2003) 5:337-350


2. PubMed ID: 17989254
   Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
   "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
   "Genome Res (2007) 17:1850-1864


3. PubMed ID: 17989255
   Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
   "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
   "Genome Res (2007) 17:1865-1879


4. PubMed ID: 12844358
   Computational identification of Drosophila microRNA genes
   "Lai EC, Tomancak P, Williams RW, Rubin GM"
   "Genome Biol (2003) 4:R42