miRBase entry: dme-mir-274

Stem-loop dme-mir-274


Accession
MI0000355
Description
Drosophila melanogaster dme-mir-274 precursor miRNA mir-274
Gene
family?
RF00822; mir-274

Literature search
5 open access papers mention dme-mir-274
(8 sentences)

Sequence

257793 reads, 2369 reads per million, 43 experiments
uccuguguugcaguuucguUUUGUGACCGACACUAACGGGUAauuguuuggccgccaggauuaCUCGUUUUUGCGAUCACAAAUUAugaaauugcagcaa
.....(((((((((((((((((((((.((.((..(((((((((((..((((...)))))))))))))))..)))).)))))))..)))))))))))))).

Structure
uccug              --       C  A  CU           gu    c 
     uguugcaguuucgu  UUUGUGA CG CA  AACGGGUAauu  uugg  
     ||||||||||||||  ||||||| || ||  |||||||||||  |||| c
     acgacguuaaaguA  AAACACU GC GU  UUGCUCauuag  gacc  
----a              UU       A  -  UU           --    g 


Annotation confidence High
Do you think this miRNA is real?
Comments
This sequence is computationally predicted based on conservation in D. pseudoobscura. The precise 5' or 3' ends are unknown. Northern blotting confirmed that the strand containing the predicted miR is predominantly expressed.

Genome context
chr3L: 11656918-11657017 [+]

Database links

Mature dme-miR-274-5p

Accession MIMAT0000332
Description Drosophila melanogaster dme-miR-274-5p mature miRNA
Sequence 20 - UUUGUGACCGACACUAACGGGUA - 42
Evidence experimental
Northern [1], 454 [2-3], Illumina [3]
Database links
Predicted targets

Mature dme-miR-274-3p

Accession MIMAT0020800
Description Drosophila melanogaster dme-miR-274-3p mature miRNA
Sequence 64 - CUCGUUUUUGCGAUCACAAAUUA - 86
Evidence not_experimental
Database links

References

  1. PubMed ID: 17989254
    Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
    "Genome Res (2007) 17:1850-1864

  2. PubMed ID: 17989255
    Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
    "Genome Res (2007) 17:1865-1879

  3. PubMed ID: 12844358
    Computational identification of Drosophila microRNA genes
    "Lai EC, Tomancak P, Williams RW, Rubin GM"
    "Genome Biol (2003) 4:R42