miRBase entry: dme-mir-316

Stem-loop dme-mir-316


Accession
MI0000428
Description
Drosophila melanogaster dme-mir-316 precursor miRNA mir-316
Gene
family?
RF00814; mir-316

Literature search
2 open access papers mention dme-mir-316
(3 sentences)

Sequence

26984 reads, 506 reads per million, 42 experiments
aaauucuagucgauuUGUCUUUUUCCGCUUACUGGCGuuucaauuccacaacgACAGGAAAGGGAAAAAGGCGUAuuuacuaugaguuu
((((((((((.(((.(((((((((((.(((.(((.((((..........)))).)))..)))))))))))))).))).)))).))))))

Structure
      -    c   u           G   -A   G    ucaa 
aaauuc uagu gau UGUCUUUUUCC CUU  CUG CGuu    u
|||||| |||| ||| ||||||||||| |||  ||| ||||     
uuugag auca uuA GCGGAAAAAGG GAA  GAC gcaa    u
      u    u   U           -   AG   A    cacc 


Annotation confidence High
Do you think this miRNA is real?
Comments
Lai et al. predicted this sequence based on conservation in D. pseudoobscura, but did not verify expression [2]. Aravin et al. describe identification and mapping of the 5' end of the excised sequence by cloning [1].

Genome context
chr3L: 21668714-21668802 [-]

Database links

Mature dme-miR-316-5p

Accession MIMAT0000408
Description Drosophila melanogaster dme-miR-316-5p mature miRNA
Sequence 16 - UGUCUUUUUCCGCUUACUGGCG - 37
Evidence experimental
cloned [1], 454 [3-4], Illumina [4]
Database links
Predicted targets

Mature dme-miR-316-3p

Accession MIMAT0020842
Description Drosophila melanogaster dme-miR-316-3p mature miRNA
Sequence 54 - ACAGGAAAGGGAAAAAGGCGUA - 75
Evidence not_experimental
Database links

References

  1. PubMed ID: 12919683
    The small RNA profile during Drosophila melanogaster development
    "Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T"
    "Dev Cell (2003) 5:337-350

  2. PubMed ID: 17989254
    Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
    "Genome Res (2007) 17:1850-1864

  3. PubMed ID: 17989255
    Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
    "Genome Res (2007) 17:1865-1879

  4. PubMed ID: 12844358
    Computational identification of Drosophila microRNA genes
    "Lai EC, Tomancak P, Williams RW, Rubin GM"
    "Genome Biol (2003) 4:R42