miRBase entry: bta-mir-122

Stem-loop bta-mir-122


Accession
MI0005063
Description
Bos taurus bta-mir-122 precursor miRNA mir-122
Gene
family?
RF00684; mir-122

Literature search
267 open access papers mention bta-mir-122
(1335 sentences)

Sequence

19801029 reads, 21536.0 reads per million, 252 experiments
ccuuagcagagcugUGGAGUGUGACAAUGGUGUUUGuguccaaacuaucaAACGCCAUUAUCACACUAAAUAGcuacuguuaggc
.((((((((((((((..(((((((.(((((((((((............))))))))))).)))))))..)))))).)))))))).

Structure
c        -      GG       C           ugucc 
 cuuagcag agcugU  AGUGUGA AAUGGUGUUUG     a
 |||||||| ||||||  ||||||| |||||||||||      
 ggauuguc ucGAUA  UCACACU UUACCGCAAac     a
c        a      AA       A           uauca 


Annotation confidence High
Do you think this miRNA is real?
Comments
The mature miR-122 sequence identified by Sonstegard et al [1] has an additional C base at the 5' end, which conflicts with the draft genome sequence.

Genome context
24: 57588139-57588223 [+]

Database links

Mature bta-miR-122-5p

Accession MIMAT0003849
Description Bos taurus bta-miR-122-5p mature miRNA
Sequence 15 - UGGAGUGUGACAAUGGUGUUUG - 36
Evidence experimental
cloned [1,3], Array [2], qRT-PCR [2]

Mature bta-miR-122-3p

Accession MIMAT0050799
Description Bos taurus bta-miR-122-3p mature miRNA
Sequence 51 - AACGCCAUUAUCACACUAAAUAG - 73
Evidence experimental
cloned [1,3], Array [2], qRT-PCR [2]

References

  1. PubMed ID: 17105755
    Discovery and profiling of bovine microRNAs from immune-related and embryonic tissues
    "Coutinho LL, Matukumalli LK, Sonstegard TS, Van Tassell CP, Gasbarre LC, Capuco AV, Smith TP"
    "Physiol Genomics (2007) 29:35-43

  2. PubMed ID: 19267191
    Identification and characteristics of cattle microRNAs by homology searching and small RNA cloning
    "Long JE, Chen HX"
    "Biochem Genet (2009) 47:329-343

  3. PubMed ID: 19170227
    Identification and expression profiling of microRNAs during bovine oocyte maturation using heterologous approach
    "Tesfaye D, Worku D, Rings F, Phatsara C, Tholen E, Schellander K, Hoelker M"
    "Mol Reprod Dev (2009) 76:665-677