Stem-loop cre-MIR905

Accession

MI0005698

Description

Chlamydomonas reinhardtii cre-MIR905 precursor miRNA

Sequence

ugccgggcuggggccgggcgggcugugaucgaccuggAGGUCCCUGGAUAUGGCACCuucgagguuguggucacacacucaguccggggaccucggcccaguugcuggugcuaugcucugacuacccgacaagcaagcuuggggccgccccccacauugguccugcgguagucagaacagagcacuagcgacugggccgagguaacccgcacugagugcgugaccacaaccucgAAGGUGCCAUAUCCAGGGACCuccaggucgaucacagcccgcccggccccagcgcggca
(((((.((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((((.((((((((.((((.(((((((((((((((((((((((.((.((((((((((..(..(.(((...(((((.....)))))..))).)..)..)))))))))).)).)))))))))))))))))))))))..)))).)))))))).)))))))))))))))))))))))))))))))))))))))))))))))))))))))))))))))))))).)))))

Structure

     g                                                                    a        c    -g                       a  c          cg ca g   gcu     c 
ugccg gcuggggccgggcgggcugugaucgaccuggAGGUCCCUGGAUAUGGCACCuucgagguuguggucac cacucagu cggg  accucggcccaguugcuggugcu ug ucugacuacc  a  a caa   ugggg c
||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||| ||||  ||||||||||||||||||||||| || ||||||||||  |  | |||   ||||| g
acggc cgaccccggcccgcccgacacuagcuggaccuCCAGGGACCUAUACCGUGGAAgcuccaacaccagug gugaguca gccc  uggagccgggucagcgaucacga ac agacugaugg  u  u guu   acccc c
     g                                                                    c        c    aa                       g  a          cg cc g   -ac     c 

Annotation confidence Not enough data

Comments

Molner et al. and Zhao et al agree that the predominant product from this hairpin precursor is from the 3' arm [1,2]. The product shown here is as described in [2], offset slightly from that described in [1]. Molner et al also identify a number of further offset and overlapping minor products, not shown here [2].

Genome context

scaffold_75: 370850-371142 [+]

Mature cre-miR905-5p

• Accession: MIMAT0004964
• Description: Chlamydomonas reinhardtii cre-miR905-5p mature miRNA
• Sequence: 38 - AGGUCCCUGGAUAUGGCACC - 57
• Evidence: experimental ; cloned [2]

Mature cre-miR905-3p

• Accession: MIMAT0004386
• Description: Chlamydomonas reinhardtii cre-miR905-3p mature miRNA
• Sequence: 235 - AAGGUGCCAUAUCCAGGGACC - 255
• Evidence: experimental ; cloned [1-2]

References

1. PubMed ID: 17470535
   A complex system of small RNAs in the unicellular green alga Chlamydomonas reinhardtii
   "Zhao T, Li G, Mi S, Li S, Hannon GJ, Wang XJ, Qi Y"
   "Genes Dev (2007) 21:1190-1203


2. PubMed ID: 17538623
   miRNAs control gene expression in the single-cell alga Chlamydomonas reinhardtii
   "Molnar A, Schwach F, Studholme DJ, Thuenemann EC, Baulcombe DC"
   "Nature (2007) 447:1126-1129