miRBase entry: cre-MIR909

Stem-loop cre-MIR909


Accession
MI0005702
Description
Chlamydomonas reinhardtii cre-MIR909 precursor miRNA


Sequence

93486 reads, 4824.0 reads per million, 51 experiments
guucccgcacacguggcugaaggucgcggagcucuuugccgacacgguggccauguggucccgcagcuccuugccacacgggcaugcaaacaugacccugaauggcccugccugcggccugccccgcuccagggccuucugcugcUGAUGGUGCAGAUGCGGCUGGugcuggucaaaccgguggugguggugcuggugcuguucgggcuccugcugcaccggcaguagcgccagcagcuggagcagguacagggcggccgccagcggcagcaccaccaccaccaccaccgguucgaccagcaccAGCCGCACCUGCACCACCAGCagcggauagaaggcuccggagcggggcaggcccaggcagggccauucagggucaaguuugcaugcccguguggcaagacgcugcgguaccacauggccaccgugucggcgaagggcuccgugaccuucagccacuugugcgggaac
(((((((((((.(((((((((((((((((((((((((((((((((((((((((((((((.(((((((..((((((((((((((((((((((.((((((((((((((((((((((.(((((((((((((((.((((((((((((((((.((((((((.(((((((((((((((((.(((((((((((((((((.(((((((((.((.(((((.(((((...(((......))).))))).)))))........(((....)))..))))))))))).))))))))))))))))).))))))))))))))))).)))))))).)))))))....))))))))).))))))))))))))))))))))))))))))))))))).))))))))))))))))))))))..))))))).))))))))))))))))))))))))))))))))))))))))))))))).)))))))))))

Structure
           c                                               c       uc                      a                      c               a         ----       A        A                 a                 c         u  -------------------g     u     acc   ag 
guucccgcaca guggcugaaggucgcggagcucuuugccgacacgguggccauguggu ccgcagc  cuugccacacgggcaugcaaac ugacccugaauggcccugccug ggccugccccgcucc gggccuucu    gcugcUG UGGUGCAG UGCGGCUGGugcugguc aaccgguggugguggug uggugcugu cg                    gcucc gcugc   ggc  u
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| |||||||  |||||||||||||||||||||| |||||||||||||||||||||| ||||||||||||||| |||||||||    ||||||| |||||||| ||||||||||||||||| ||||||||||||||||| ||||||||| ||                    ||||| |||||   |||   
caagggcgugu caccgacuuccagugccucgggaagcggcugugccaccgguacacca ggcgucg  gaacggugugcccguacguuug acugggacuuaccgggacggac ccggacggggcgagg cucggaaga    cgaCGAC ACCACGUC ACGCCGAccacgaccag uuggccaccaccaccac accacgacg gc                    cgagg cgacg   ccg  a
           u                                               u       ca                      a                      -               c         uagg       C        C                 c                 c         -  gaccgccggcgggacaugga     u     --a   cg 


Annotation confidence Medium
Do you think this miRNA is real?
Comments
Zhao et al. identify a pair of mature miRNA product from the 5' and 3' arms of the hairpin precursor, and named them miR909-5p and miR909-3p [1]. Molner et al. suggest that an alternative 5' product is the predominant one, named cre-miR909.1 here [2]. The Zhao et al. products are renamed miR909.2 and miR909.3 here. Molner et al. also show a number of offset and overlapping minor products originating from the same hairpin [2], but not catalogued here.

Genome context
Unknown

Database links

Mature cre-miR909-5p

Accession MIMAT0004967
Description Chlamydomonas reinhardtii cre-miR909-5p mature miRNA
Sequence 146 - UGAUGGUGCAGAUGCGGCUGG - 166
Evidence experimental
cloned [2]

Mature cre-miR909-3p

Accession MIMAT0004391
Description Chlamydomonas reinhardtii cre-miR909-3p mature miRNA
Sequence 305 - AGCCGCACCUGCACCACCAGC - 325
Evidence experimental
cloned [1-2]

References

  1. PubMed ID: 17470535
    A complex system of small RNAs in the unicellular green alga Chlamydomonas reinhardtii
    "Zhao T, Li G, Mi S, Li S, Hannon GJ, Wang XJ, Qi Y"
    "Genes Dev (2007) 21:1190-1203

  2. PubMed ID: 17538623
    miRNAs control gene expression in the single-cell alga Chlamydomonas reinhardtii
    "Molnar A, Schwach F, Studholme DJ, Thuenemann EC, Baulcombe DC"
    "Nature (2007) 447:1126-1129