Stem-loop cre-MIR912

Accession

MI0005705

Description

Chlamydomonas reinhardtii cre-MIR912 precursor miRNA

Sequence

gccuaugccucugccggccaucaauggcgcucacgacaagggugccaucgucaccggucuaaucuaaugccaggcaaccagggaagacacaggauccauuuucuagugccuggcugggaucaaugcaagaugauaguuggauccuggacgccgaaggccaucaugcggugucaaccgcaugacggccguuggcguccaggaucuagcugucgucuUGGAUUGAUCCCAGCCAGGCgcuuggagauggacccacggucuucccucccuuguugccuggguuccgagcagaccggcgacgauggcaccaccgucguuggcuccagggcauugacggcccggcggagaaccaggc
((((....(((((((((((.((((((.((((.(((((...((((((((((((.(((((((..((.....(((((((((.(((((((((...((.(((((((((.((((((((((((((((((((.(((((((((((((((((((((((((((((.((((.(((((((((....))))))))).)))).))))))))))))))))))))))))))))).)))))))))))))))))))).))))))))).))...))))))))).....))))))))).....))..))))))).))))))))))))...))))).))).....).)))))).)).)))))))))....))))

Structure

    augc         -  a      g -----   c     aag            a       aa  uaaug         ----c         aca  a         u                    g                             a    a         g 
gccu    cucugccgg cc ucaaug c     gcu acgac   ggugccaucguc ccggucu  uc     ccaggcaac     agggaagac   gg uccauuuuc agugccuggcugggaucaau caagaugauaguuggauccuggacgccga ggcc ucaugcggu u
||||    ||||||||| || |||||| |     ||| |||||   |||||||||||| |||||||  ||     |||||||||     |||||||||   || ||||||||| |||||||||||||||||||| ||||||||||||||||||||||||||||| |||| |||||||||  
cgga    gaggcggcc gg aguuac g     cgg ugcug   ccacgguagcag ggccaga  ag     gguccguug     ucccuucug   cc agguagagg ucgCGGACCGACCCUAGUUA GUucugcugucgaucuaggaccugcgguu ccgg aguacgcca c
    ccaa         c  c      g gaccu   u     cca            c       cg  ccuug         uuccc         gca  c         u                    G                             g    c         a 

Annotation confidence Not enough data

Comments

Molner et al. confirm that the mature miRNA product identified by Zhao et al. and shown here is the predominant one [2]. They also identify 3 further minor products from the same hairpin, not shown here.

Genome context

scaffold_35: 929764-930115 [-]

Mature cre-miR912

• Accession: MIMAT0004395
• Description: Chlamydomonas reinhardtii cre-miR912 mature miRNA
• Sequence: 216 - UGGAUUGAUCCCAGCCAGGC - 235
• Evidence: experimental ; cloned [1-2]

References

1. PubMed ID: 17470535
   A complex system of small RNAs in the unicellular green alga Chlamydomonas reinhardtii
   "Zhao T, Li G, Mi S, Li S, Hannon GJ, Wang XJ, Qi Y"
   "Genes Dev (2007) 21:1190-1203


2. PubMed ID: 17538623
   miRNAs control gene expression in the single-cell alga Chlamydomonas reinhardtii
   "Molnar A, Schwach F, Studholme DJ, Thuenemann EC, Baulcombe DC"
   "Nature (2007) 447:1126-1129