Stem-loop aae-mir-998

Accession

MI0013444

Description

Aedes aegypti aae-mir-998 precursor miRNA

Literature search

3 open access papers mention aae-mir-998
(5 sentences)

Sequence

1271711 reads, 4552.0 reads per million, 88 experiments

aagaaccaauacccgaACUGAACUCUCGUGGGUCUGCAguugauauuucgaaagcuugUAGCACCAUGAGAUUCAGCUCcgguguugguucg
..(((((((((((.((.(((((.((((((((..((((((((((....))))....))))))..))))))))))))).)).))))))))))).

Structure

aa           c  A     C        GU      ----    u 
  gaaccaauacc ga CUGAA UCUCGUGG  CUGCAg    uuga a
  ||||||||||| || ||||| ||||||||  ||||||    ||||  
  cuugguugugg CU GACUU AGAGUACC  GAUguu    agcu u
-g           c  C     -        AC      cgaa    u 

Annotation confidence High

Comments

This miRNA was identified independently in Aedes aegypti [1] and in the closely related Aedes albopictus, for which there was no genome sequence [2]. The latter reads were therefore also mapped to the Aedes aegypti genome [2].

Genome context

ref|NW_001811512.1|: 322280-322371 [+]

Clustered miRNAs

1 other miRNA is < 10 kb from aae-mir-998

Mature aae-miR-998-5p

• Accession: MIMAT0057322
• Description: Aedes aegypti aae-miR-998-5p mature miRNA
• Sequence: 17 - ACUGAACUCUCGUGGGUCUGCA - 38
• Evidence: experimental ; 454 [1], Illumina [2]

Mature aae-miR-998-3p

• Accession: MIMAT0014227
• Description: Aedes aegypti aae-miR-998-3p mature miRNA
• Sequence: 59 - UAGCACCAUGAGAUUCAGCUC - 79
• Evidence: experimental ; 454 [1], Illumina [2]

References

1. PubMed ID: 19961592
   Direct sequencing and expression analysis of a large number of miRNAs in Aedes aegypti and a multi-species survey of novel mosquito miRNAs
   Li S, Mead EA, Liang S, Tu Z
   BMC Genomics (2009) 10:581


2. PubMed ID: 20167119
   Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus
   Skalsky RL, Vanlandingham DL, Scholle F, Higgs S, Cullen BR
   BMC Genomics (2010) 11:119