Stem-loop aae-mir-996

Accession

MI0013460

Description

Aedes aegypti aae-mir-996 precursor miRNA

Literature search

4 open access papers mention aae-mir-996
(6 sentences)

Sequence

234260 reads, 998.0 reads per million, 79 experiments

aacucaauuuucgucgGCGUGCAUGAAUCUGGUGCAcgguuuuccaguuguaauaucgcuucgUGACUAGAUUACAUGCUCGUCUaugaaguugagau
..((((((((.(((.((((.(((((((((((((.(((((.....................))))))))))))).))))).)))).)))))))))))..

Structure

aa        u   c    U     -        G     uuuuccagu 
  cucaauuu cgu gGCG GCAUG AAUCUGGU CAcgg         u
  |||||||| ||| |||| ||||| |||||||| |||||         g
  gaguugaa gua CUGC CGUAC UUAGAUCA GUgcu         u
ua        -   U    U     A        -     ucgcuauaa 

Annotation confidence High

Comments

This miRNA was identified independently in Aedes aegypti [1] and in the closely related Aedes albopictus, for which there was no genome sequence [2]. The latter reads were therefore also mapped to the Aedes aegypti genome [2].

Genome context

ref|NW_001811171.1|: 567493-567590 [-]

Clustered miRNAs

1 other miRNA is < 10 kb from aae-mir-996

Mature aae-miR-996-5p

• Accession: MIMAT0057329
• Description: Aedes aegypti aae-miR-996-5p mature miRNA
• Sequence: 17 - GCGUGCAUGAAUCUGGUGCA - 36
• Evidence: experimental ; 454 [1], Illumina [2]

Mature aae-miR-996-3p

• Accession: MIMAT0014251
• Description: Aedes aegypti aae-miR-996-3p mature miRNA
• Sequence: 64 - UGACUAGAUUACAUGCUCGUCU - 85
• Evidence: experimental ; 454 [1], Illumina [2]

References

1. PubMed ID: 19961592
   Direct sequencing and expression analysis of a large number of miRNAs in Aedes aegypti and a multi-species survey of novel mosquito miRNAs
   Li S, Mead EA, Liang S, Tu Z
   BMC Genomics (2009) 10:581


2. PubMed ID: 20167119
   Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus
   Skalsky RL, Vanlandingham DL, Scholle F, Higgs S, Cullen BR
   BMC Genomics (2010) 11:119