Stem-loop aae-mir-999

Accession

MI0013464

Description

Aedes aegypti aae-mir-999 precursor miRNA

Literature search

2 open access papers mention aae-mir-999
(2 sentences)

Sequence

1081893 reads, 6687.0 reads per million, 88 experiments

gcagggACGCGGUCGUGCAGGAAAUUAUUUggcauuagauucaaaUGUUAACUGUAAGACUGUGUCUUugcgggugauc
((((((((((((((.(((((..(((.((((((........)))))))))..))))).))))))))))))))........

Structure

--------              G     GA   U      cau 
        gcagggACGCGGUC UGCAG  AAU AUUUgg   u
        |||||||||||||| |||||  ||| ||||||    
        cguUUCUGUGUCAG AUGUC  UUG Uaaacu   a
cuaguggg              A     AA   -      uag 

Annotation confidence High

Comments

This miRNA was identified independently in Aedes aegypti [1] and in the closely related Aedes albopictus, for which there was no genome sequence [2]. The latter reads were therefore also mapped to the Aedes aegypti genome [2].

Genome context

ref|NW_001809803.1|: 2315100-2315178 [+]

Mature aae-miR-999-5p

• Accession: MIMAT0061500
• Description: Aedes aegypti aae-miR-999-5p mature miRNA
• Sequence: 7 - ACGCGGUCGUGCAGGAAAUUAUUU - 30
• Evidence: experimental ; 454 [1], Illumina [2]

Mature aae-miR-999-3p

• Accession: MIMAT0014257
• Description: Aedes aegypti aae-miR-999-3p mature miRNA
• Sequence: 46 - UGUUAACUGUAAGACUGUGUCUU - 68
• Evidence: experimental ; 454 [1], Illumina [2]

References

1. PubMed ID: 19961592
   Direct sequencing and expression analysis of a large number of miRNAs in Aedes aegypti and a multi-species survey of novel mosquito miRNAs
   Li S, Mead EA, Liang S, Tu Z
   BMC Genomics (2009) 10:581


2. PubMed ID: 20167119
   Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus
   Skalsky RL, Vanlandingham DL, Scholle F, Higgs S, Cullen BR
   BMC Genomics (2010) 11:119