Stem-loop aae-mir-309a-2

Accession

MI0013478

Description

Aedes aegypti aae-mir-309a-2 precursor miRNA

Literature search

2 open access papers mention aae-mir-309a-2
(3 sentences)

Sequence

10570 reads, 86.0 reads per million, 68 experiments

uccaccgaauguuaugCGACUAACUUCGUUCAGUUUGGUGucauacgaagguuuccaguuucaUCACUGGGCAAAGUUUGUCGCauaaaug
...........(((((((((.(((((.(((((((..((((..((..(((...)))..))..))))))))))).))))).)))))))))...

Structure

uccaccgaaug         U     C       UU    uc  ac   g 
           uuaugCGAC AACUU GUUCAGU  GGUG  au  gaa  
           ||||||||| ||||| |||||||  ||||  ||  ||| g
           aauaCGCUG UUGAA CGGGUCA  CUac  ug  cuu  
--------gua         U     A       --    uu  ac   u 

Annotation confidence High

Comments

This miRNA was experimentally identified in the closely related Aedes albopictus in [2], for which there was no genome sequence, and therefore mapped to the Aedes aegypti genome. Li et al. also validate the expression in A. aegypti [1].

Genome context

ref|NW_001811355.1|: 94025-94115 [+]

Clustered miRNAs

3 other miRNAs are < 10 kb from aae-mir-309a-2

Mature aae-miR-309a-5p

• Accession: MIMAT0057338
• Description: Aedes aegypti aae-miR-309a-5p mature miRNA
• Sequence: 17 - CGACUAACUUCGUUCAGUUUGGUG - 40
• Evidence: experimental ; Illumina [1], 454 [2]

Mature aae-miR-309a-3p

• Accession: MIMAT0014272
• Description: Aedes aegypti aae-miR-309a-3p mature miRNA
• Sequence: 64 - UCACUGGGCAAAGUUUGUCGC - 84
• Evidence: experimental ; Illumina [1], 454 [2]

References

1. PubMed ID: 19961592
   Direct sequencing and expression analysis of a large number of miRNAs in Aedes aegypti and a multi-species survey of novel mosquito miRNAs
   Li S, Mead EA, Liang S, Tu Z
   BMC Genomics (2009) 10:581


2. PubMed ID: 20167119
   Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus
   Skalsky RL, Vanlandingham DL, Scholle F, Higgs S, Cullen BR
   BMC Genomics (2010) 11:119