Stem-loop hsa-mir-221

Accession

MI0000298

Symbol

HGNC: MIR221

Description

Homo sapiens hsa-mir-221 precursor miRNA mir-221

Gene
family

RF00651; mir-221

Literature search

572 open access papers mention hsa-mir-221
(4115 sentences)

Sequence

1352665 reads, 5088 reads per million, 157 experiments

ugaacauccaggucuggggcaugaACCUGGCAUACAAUGUAGAUUUcuguguucguuaggcaacAGCUACAUUGUCUGCUGGGUUUCaggcuaccuggaaacauguucuc
.((((((((((((....(((.(((((((((((.(((((((((....(((((((.....))).))))))))))))).))))))).)))).))))))))))....)))))..

Structure

-u     ----       cugg   a    -       U         AUUU    -   c 
  gaaca    uccaggu    ggc ugaA CCUGGCA ACAAUGUAG    cugu guu g
  |||||    |||||||    ||| |||| ||||||| |||||||||    |||| ||| u
  cuugu    aggucca    ucg aCUU GGGUCGU UGUUACAUC    GAca cgg u
cu     acaa       ----   g    U       C         ----    a   a 

Annotation confidence High

Comments

This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences [1]. Expression of the excised miR has been validated in zebrafish, and later validated in human HL-60 leukemia cells [2].

Genome context

chrX: 45746157-45746266 [-]

Clustered miRNAs

1 other miRNA is < 10 kb from hsa-mir-221

Disease association

hsa-mir-221 is associated with one or more human diseases in the Human microRNA Disease Database

Mature hsa-miR-221-5p

• Accession: MIMAT0004568
• Description: Homo sapiens hsa-miR-221-5p mature miRNA
• Sequence: 25 - ACCUGGCAUACAAUGUAGAUUU - 46
• Evidence: experimental ; cloned [3]

Mature hsa-miR-221-3p

• Accession: MIMAT0000278
• Description: Homo sapiens hsa-miR-221-3p mature miRNA
• Sequence: 65 - AGCUACAUUGUCUGCUGGGUUUC - 87
• Evidence: experimental ; cloned [2-4], Illumina [5]

References

1. PubMed ID: 15325244
   Altered expression profiles of microRNAs during TPA-induced differentiation of HL-60 cells
   "Kasashima K, Nakamura Y, Kozu T"
   "Biochem Biophys Res Commun (2004) 322:403-410


2. PubMed ID: 17604727
   A mammalian microRNA expression atlas based on small RNA library sequencing
   "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
   "Cell (2007) 129:1401-1414


3. PubMed ID: 17616659
   Patterns of known and novel small RNAs in human cervical cancer
   "Lui WO, Pourmand N, Patterson BK, Fire A"
   "Cancer Res (2007) 67:6031-6043


4. PubMed ID: 20158877
   Analysis of deep sequencing microRNA expression profile from human embryonic stem cells derived mesenchymal stem cells reveals possible role of let-7 microRNA family in downstream targeting of hepatic nuclear factor 4 alpha
   Koh W, Sheng CT, Tan B, Lee QY, Kuznetsov V, Kiang LS, Tanavde V
   BMC Genomics (2010) 11:S6


5. PubMed ID: 12624257
   Vertebrate microRNA genes
   "Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP"
   "Science (2003) 299:1540